Efficient optogenetic silencing of neurotransmitter release with a mosquito rhodopsin.
Animals
Cells, Cultured
Culicidae
Dopamine
/ metabolism
Dopaminergic Neurons
/ metabolism
HEK293 Cells
Humans
Insect Proteins
/ genetics
Locomotion
Mice
Mice, Inbred C57BL
Optogenetics
/ methods
Rats
Rats, Sprague-Dawley
Rats, Wistar
Rhodopsin
/ genetics
Substantia Nigra
/ cytology
Synaptic Potentials
G protein-coupled receptor
GCPR
autaptic neurons
dopaminergic
eOPN3
inhibitory
mosquito
optogenetics
presynaptic
silencing
thalamocortical
Journal
Neuron
ISSN: 1097-4199
Titre abrégé: Neuron
Pays: United States
ID NLM: 8809320
Informations de publication
Date de publication:
19 05 2021
19 05 2021
Historique:
received:
02
02
2021
revised:
02
03
2021
accepted:
09
03
2021
pubmed:
13
5
2021
medline:
22
6
2021
entrez:
12
5
2021
Statut:
ppublish
Résumé
Information is carried between brain regions through neurotransmitter release from axonal presynaptic terminals. Understanding the functional roles of defined neuronal projection pathways requires temporally precise manipulation of their activity. However, existing inhibitory optogenetic tools have low efficacy and off-target effects when applied to presynaptic terminals, while chemogenetic tools are difficult to control in space and time. Here, we show that a targeting-enhanced mosquito homolog of the vertebrate encephalopsin (eOPN3) can effectively suppress synaptic transmission through the G
Identifiants
pubmed: 33979634
pii: S0896-6273(21)00161-6
doi: 10.1016/j.neuron.2021.03.013
pmc: PMC7611984
mid: EMS138041
pii:
doi:
Substances chimiques
Insect Proteins
0
Rhodopsin
9009-81-8
Dopamine
VTD58H1Z2X
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1621-1635.e8Subventions
Organisme : European Research Council
ID : 714762
Pays : International
Commentaires et corrections
Type : CommentIn
Type : CommentIn
Informations de copyright
Copyright © 2021 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests O.Y. and M.M. have disclosed these findings to Yeda, the Weizmann Institute Technology Transfer Arm, which is filing a patent application on these developments. The constructs and viral vectors remain freely available from the authors and through Addgene.
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