Improving in vitro ciguatoxin and brevetoxin detection: selecting neuroblastoma (Neuro-2a) cells with lower sensitivity to ouabain and veratridine (OV-LS).

Animals Cell Line, Tumor Ciguatoxins / toxicity Marine Toxins / toxicity Neuroblastoma Ouabain Oxocins Veratridine
Ciguatera poisoning Ciguatoxin, brevetoxin Marine biotoxin Marine toxin detection Neuro-2a cell-based assay Neurotoxic shellfish poisoning

Journal

Harmful algae
ISSN: 1878-1470
Titre abrégé: Harmful Algae
Pays: Netherlands
ID NLM: 101128968

Informations de publication

Date de publication:
03 2021
Historique:
received: 10 01 2020
revised: 31 12 2020
accepted: 25 01 2021
entrez: 13 5 2021
pubmed: 14 5 2021
medline: 28 5 2021
Statut: ppublish

Résumé

Marine biotoxins accumulating in seafood products pose a risk to human health. These toxins are often potent in minute amounts and contained within complex matrices; requiring sensitive, reliable, and robust methods for their detection. The mouse neuroblastoma (Neuro-2a) cytotoxicity assay (N2a-assay) is a sensitive, high-throughput, in vitro method effective for detecting sodium channel-specific marine biotoxins. The N2a-assay can be conducted to distinguish between specific effects on voltage-gated sodium (Na

Identifiants

DOI: 10.1016/j.hal.2021.101994 PMID: 33980434
pubmed: 33980434
pii: S1568-9883(21)00021-4
doi: 10.1016/j.hal.2021.101994
pii:
doi:

Substances chimiques

Marine Toxins 0
Oxocins 0
Ciguatoxins 11050-21-8
Ouabain 5ACL011P69
Veratridine 71-62-5
brevetoxin 98225-48-0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

101994

Informations de copyright

Copyright © 2021. Published by Elsevier B.V.

Auteurs

Christopher R Loeffler (CR)

German Federal Institute for Risk Assessment, Department Safety in the Food Chain, National Reference Laboratory of Marine Biotoxins, Max-Dohrn-Str. 8-10, 10589 Berlin, Germany - www.bfr.bund.de; Department of Pharmacy, School of Medicine and Surgery, University of Napoli Federico II, Via D. Montesano 49, 80131, Napoli, Italy. Electronic address: Christopher.Loeffler@bfr.bund.de.

Dorina Bodi (D)

German Federal Institute for Risk Assessment, Department Safety in the Food Chain, National Reference Laboratory of Marine Biotoxins, Max-Dohrn-Str. 8-10, 10589 Berlin, Germany - www.bfr.bund.de.

Luciana Tartaglione (L)

Department of Pharmacy, School of Medicine and Surgery, University of Napoli Federico II, Via D. Montesano 49, 80131, Napoli, Italy; CoNISMa - Italian Interuniversity Consortium on Marine Sciences, Piazzale Flaminio 9, 00196 Rome, Italy.

Carmela Dell'Aversano (C)

Department of Pharmacy, School of Medicine and Surgery, University of Napoli Federico II, Via D. Montesano 49, 80131, Napoli, Italy; CoNISMa - Italian Interuniversity Consortium on Marine Sciences, Piazzale Flaminio 9, 00196 Rome, Italy.

Angelika Preiss-Weigert (A)

German Federal Institute for Risk Assessment, Department Safety in the Food Chain, National Reference Laboratory of Marine Biotoxins, Max-Dohrn-Str. 8-10, 10589 Berlin, Germany - www.bfr.bund.de.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Improving in vitro ciguatoxin and brevetoxin...
questionsmedicales.fr › Cellules › Cellules cultivées › Cellules cancéreuses en culture › Lignée cellulaire tumorale › Improving in vitro ciguatoxin and brevetoxin...
questionsmedicales.fr › Facteurs biologiques › Toxines biologiques › Toxines de la flore et de la faune marines › Ciguatoxines › Improving in vitro ciguatoxin and brevetoxin...
questionsmedicales.fr › Facteurs biologiques › Toxines biologiques › Toxines de la flore et de la faune marines › Improving in vitro ciguatoxin and brevetoxin...
questionsmedicales.fr › Tumeurs › Tumeurs par type histologique › Tumeurs du tissu nerveux › Tumeurs neuroectodermiques › Tumeurs neuroépitheliales › Tumeurs neuroectodermiques primitives › Tumeurs neuroectodermiques primitives périphériques › Neuroblastome › Improving in vitro ciguatoxin and brevetoxin...
questionsmedicales.fr › Glucides › Hétérosides › Glucosides cardiotoniques › Strophantines › Ouabaïne › Improving in vitro ciguatoxin and brevetoxin...
questionsmedicales.fr › Composés hétérocycliques › Composés hétéromonocycliques › Oxocines › Improving in vitro ciguatoxin and brevetoxin...
questionsmedicales.fr › Composés hétérocycliques › Composés hétérocycliques à cycles fusionnés › Composés hétérocycliques avec 4 noyaux ou plus › Cévanes › Vératridine › Improving in vitro ciguatoxin and brevetoxin...