Development of a PCR Lateral Flow Assay for Rapid Detection of Bacillus anthracis, the Causative Agent of Anthrax.
Anthrax
/ blood
Antigens, Bacterial
/ genetics
Bacillus anthracis
/ genetics
Bacterial Toxins
/ genetics
Bacteriological Techniques
/ methods
Chromatography, Affinity
DNA, Bacterial
/ genetics
Genome, Bacterial
/ genetics
Humans
Limit of Detection
Plasmids
/ genetics
Point-of-Care Testing
Polymerase Chain Reaction
Anthrax
B. anthracis
Edema factor
Lateral flow strips
PCR
Journal
Molecular biotechnology
ISSN: 1559-0305
Titre abrégé: Mol Biotechnol
Pays: Switzerland
ID NLM: 9423533
Informations de publication
Date de publication:
Aug 2021
Aug 2021
Historique:
received:
08
04
2021
accepted:
06
05
2021
pubmed:
14
5
2021
medline:
21
12
2021
entrez:
13
5
2021
Statut:
ppublish
Résumé
Bacillus anthracis, the causative agent of anthrax is one of the most potent listed biological warfare agents. The conventional microbiological methods of its detection are labor intensive and time consuming, whereas molecular assays are fast, sensitive and specific. PCR is one of the most reliable diagnostic tools in molecular biology. The combination of PCR with lateral flow strips can reduce the diagnostic/detection time. It gives an alternative to gel electrophoresis and offers easy and clear interpretation of results. In the present study, a PCR Lateral flow (PCR-LF) assay targeting cya gene present on pXO1 plasmid of B. anthracis has been developed. The forward and reverse primers were tagged with 6-carboxyflourescein (6-FAM) and biotin, respectively, at 5' end. The dual labeled PCR products were detected using lateral flow (LF) strips developed in this study. The PCR-LF assay could detect ≥ 5 pg of genomic DNA and ≥ 500 copies of target DNA harboured in a recombinant plasmid. The assay was able to detect as few as 10
Identifiants
pubmed: 33982268
doi: 10.1007/s12033-021-00335-6
pii: 10.1007/s12033-021-00335-6
doi:
Substances chimiques
Antigens, Bacterial
0
Bacterial Toxins
0
DNA, Bacterial
0
anthrax toxin
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
702-709Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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