Discrete limbal epithelial stem cell populations mediate corneal homeostasis and wound healing.
cornea
immune cells
limbal stem cells
limbus
lineage tracing
mathematical modeling
niche
single cell RNA sequencing
stem cell dynamics
Journal
Cell stem cell
ISSN: 1875-9777
Titre abrégé: Cell Stem Cell
Pays: United States
ID NLM: 101311472
Informations de publication
Date de publication:
01 07 2021
01 07 2021
Historique:
received:
07
07
2020
revised:
21
12
2020
accepted:
01
04
2021
pubmed:
14
5
2021
medline:
5
8
2021
entrez:
13
5
2021
Statut:
ppublish
Résumé
The accessibility and transparency of the cornea permit robust stem cell labeling and in vivo cell fate mapping. Limbal epithelial stem cells (LSCs) that renew the cornea are traditionally viewed as rare, slow-cycling cells that follow deterministic rules dictating their self-renewal or differentiation. Here, we combined single-cell RNA sequencing and advanced quantitative lineage tracing for in-depth analysis of the murine limbal epithelium. These analysis revealed the co-existence of two LSC populations localized in separate and well-defined sub-compartments, termed the "outer" and "inner" limbus. The primitive population of quiescent outer LSCs participates in wound healing and boundary formation, and these cells are regulated by T cells, which serve as a niche. In contrast, the inner peri-corneal limbus hosts active LSCs that maintain corneal epithelial homeostasis. Quantitative analyses suggest that LSC populations are abundant, following stochastic rules and neutral drift dynamics. Together these results demonstrate that discrete LSC populations mediate corneal homeostasis and regeneration.
Identifiants
pubmed: 33984282
pii: S1934-5909(21)00160-0
doi: 10.1016/j.stem.2021.04.003
pmc: PMC8254798
mid: NIHMS1692692
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1248-1261.e8Subventions
Organisme : NEI NIH HHS
ID : R21 EY030250
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2021 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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