Enhanced water dispersibility and permeability through a Caco-2 cell monolayer of β-cryptoxanthin extracted from kumquats by complexation with casein.


Journal

Food chemistry
ISSN: 1873-7072
Titre abrégé: Food Chem
Pays: England
ID NLM: 7702639

Informations de publication

Date de publication:
30 Oct 2021
Historique:
received: 16 12 2020
revised: 14 04 2021
accepted: 15 04 2021
pubmed: 14 5 2021
medline: 25 6 2021
entrez: 13 5 2021
Statut: ppublish

Résumé

β-Cryptoxanthin (BCX) possesses potential therapeutic and health benefits. However, BCX absorption is low because of its poor aqueous solubility. In this study, a complex between BCX and casein (Cas) was prepared to improve the water dispersibility and bioavailability of BCX. BCX was recovered quantitatively from freeze-dried kumquat powder through solid-liquid extraction and saponification. The complexation significantly improved the apparent solubility of BCX under acidic and neutral conditions. A cell membrane permeation test using a Caco-2 cell monolayer was performed to evaluate the bioavailability of the BCX-Cas complex. This complex and a blank sample were digested in vitro and added to the apical side of the Caco-2 cell membrane. The quantity of BCX that permeated using the BCX-Cas complex after 24 h was 22.7 times greater than that of the blank. Thus, complexation of BCX with Cas improved dramatically the bioavailability of BCX from a kumquat extract.

Identifiants

pubmed: 33984564
pii: S0308-8146(21)00828-1
doi: 10.1016/j.foodchem.2021.129822
pii:
doi:

Substances chimiques

Beta-Cryptoxanthin 0
Caseins 0
Plant Extracts 0
Water 059QF0KO0R

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

129822

Informations de copyright

Copyright © 2021 Elsevier Ltd. All rights reserved.

Auteurs

Tatsuya Oshima (T)

Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan. Electronic address: oshimat@cc.miyazaki-u.ac.jp.

Koki Takahashi (K)

Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan.

Asuka Inada (A)

Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan.

Masao Yamasaki (M)

Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan.

Yumi Yamasaki (Y)

Faculty of Regional Innovation, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan.

Nozomu Eto (N)

Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan.

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Classifications MeSH