In vivo method to evaluate antioxidative activity using UVA-induced carbonylated protein on human skin.
UVA irradiation
antioxidants
carbonylated proteins
in vivo human method
reactive oxygen species
Journal
Journal of cosmetic dermatology
ISSN: 1473-2165
Titre abrégé: J Cosmet Dermatol
Pays: England
ID NLM: 101130964
Informations de publication
Date de publication:
Mar 2022
Mar 2022
Historique:
revised:
22
03
2021
received:
29
12
2020
accepted:
07
05
2021
pubmed:
16
5
2021
medline:
1
3
2022
entrez:
15
5
2021
Statut:
ppublish
Résumé
Skin is continuously exposed to oxidative stress caused by reactive oxygen species (ROS) produced by the ultraviolet (UV) light, and it is important to evaluate the antioxidant activity. Carbonylated proteins (CPs) are candidate markers of oxidative modification as a result from the ROS. We aimed to develop the CP-based method to assess the efficacy of antioxidants in human skin. Ten healthy females were enrolled in the study to determine the UVA dosage for CP production, and another 10 females were included to evaluate the antioxidative activity. The stratum corneum was collected from test skin using D-Squame tape, and CPs from the SC were stained by fluorescence labeling and observed using a fluorescence microscope. CP level significantly increased with UVA irradiation from 15J/cm This study developed the simple, visual, and direct in vivo method to evaluate the antioxidative activity for products in human skin by measuring the CP level as an oxidative modification caused by UVA-induced ROS generation.
Sections du résumé
BACKGROUND
BACKGROUND
Skin is continuously exposed to oxidative stress caused by reactive oxygen species (ROS) produced by the ultraviolet (UV) light, and it is important to evaluate the antioxidant activity. Carbonylated proteins (CPs) are candidate markers of oxidative modification as a result from the ROS. We aimed to develop the CP-based method to assess the efficacy of antioxidants in human skin.
METHODS
METHODS
Ten healthy females were enrolled in the study to determine the UVA dosage for CP production, and another 10 females were included to evaluate the antioxidative activity. The stratum corneum was collected from test skin using D-Squame tape, and CPs from the SC were stained by fluorescence labeling and observed using a fluorescence microscope.
RESULTS
RESULTS
CP level significantly increased with UVA irradiation from 15J/cm
CONCLUSION
CONCLUSIONS
This study developed the simple, visual, and direct in vivo method to evaluate the antioxidative activity for products in human skin by measuring the CP level as an oxidative modification caused by UVA-induced ROS generation.
Substances chimiques
Antioxidants
0
Reactive Oxygen Species
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1263-1269Informations de copyright
© 2021 Wiley Periodicals LLC.
Références
Dalle-Donne I, Giustarini D, Colombo R, Rossi R, Milzani A. Protein carbonylation in human diseases. Trends Mol Med. 2003;9:169-176.
Nakashima Y, Ohta S, Wolf AM. Blue light-induced oxidative stress in live skin. Free Radic Biol Med. 2017;108:300-310.
Chakravarti B, Chakravarti DN. Oxidative modification of proteins: age-related changes. Gerontology. 2007;53:128-139.
Mudiyanselage SE, Elsner P, Thiele JJ, Hamburger M. Ultraviolet A induces generation of squalene monohydroperoxide isomers in human sebum and skin surface lipids in vitro and in vivo. J Invest Dermatol. 2003;120:915-922.
Mizutani T, Sumida H, Sagawa Y, Okano Y, Masaki H. Carbonylated proteins exposed to UVA and to blue light generate reactive oxygen species through a type I photosensitizing reaction. J Dermatol Sci. 2016;84:314-321.
Iwai I, Shimadzu K, Kobayashi Y, Hirao T, Etou T. Increased carbonyl protein level in the stratum corneum of inflammatory skin disorders: A non-invasive approach. J Dermatol. 2010;37:693-698.
Yokota M, Shimizu K, Kyotani D, et al. The possible involvement of skin dryness on alterations of the dermal matrix. Exp Dermatol. 2014;23:27-31.
Kobayashi Y, Iwai I, Akutsu N, Hirao T. Increased carbonyl protein levels in the stratum corneum of the face during winter. Int J Cosmet Sci. 2008;30:35-40.
Ogura Y, Kuwahara T, Akiyama M, et al. Dermal carbonyl modification is related to the yellowish color change of photo-aged Japanese facial skin. J Dermatol Sci. 2011;64:45-52.
Masaki H, Mizutani T, Ogawa N, et al. Carbonylated proteins contribute to the darkness around facial pores. J Dermatol Sci. 2018;89:299-301.
Thiele JJ, Hsieh SN, Briviba K, Sies H. Protein oxidation in human stratum corneum: susceptibility of keratins to oxidation in vitro and presence of a keratin oxidation gradient in vivo. J Invest Dermatol. 1999;113:335-339.
Sander CS, Chang H, Salzmann S, et al. Photoaging is associated with protein oxidation in human skin in vivo. J Invest Dermatol. 2002;118(4):618-625.
Jung T, Engels M, Kaiser B, Poppek D, Grune T. Intracellular distribution of oxidized proteins and proteasome in HT22 cells during oxidative stress. Free Radic Biol Med. 2006;40:1303-1312.
Jung T, Höhn A, Catalgol B, Grune T. Age-related differences in oxidative protein-damage in young and senescent fibroblasts. Arch Biochem Biophys. 2009;483:127-135.
Fujita H, Hirao T, Takahashi M. A simple and non-invasive visualization for assessment of carbonylated protein in the stratum corneum. Skin Res Technol. 2007;13:84-90.
Sajo MEJ, Kim CS, Kim SK, et al. Antioxidant and Anti-Inflammatory Effects of Shungite against Ultraviolet B Irradiation-Induced Skin Damage in Hairless Mice. Oxid Med Cell Longev. 2017;2017:7340143.
Hossain H, Karmakar UK, Biswas SK, et al. Antinociceptive and antioxidant potential of the crude ethanol extract of the leaves of Ageratum conyzoides grown in Bangladesh. Pharm Biol. 2013;51:893-898.
Roy MK, Koide M, Rao TP, et al. ORAC and DPPH assay comparison to assess antioxidant capacity of tea infusions: relationship between total polyphenol and individual catechin content. Int J Food Sci Nutr. 2010;61:109-124.
Chandra S, Khan S, Avula B, et al. Assessment of total phenolic and flavonoid content, antioxidant properties, and yield of aeroponically and conventionally grown leafy vegetables and fruit crops: a comparative study. Evid Based Complement Alternat Med. 2014;2014:253875.
Kumar MS, Chaudhury S, Balachandran S. In vitro callus culture of Heliotropium indicum Linn. for assessment of total phenolic and flavonoid content and antioxidant activity. Appl Biochem Biotechnol. 2014;174:2897-2909.
Arakane K, Ryu A, Hayashi C, et al. Singlet oxygen (1 delta g) generation from coproporphyrin in Propionibacterium acnes on irradiation. Biochem Biophys Res Commun. 1996;223:578-582.
Ou-Yang H, Stamatas G, Saliou C, Kollias N. A chemiluminescence study of UVA-induced oxidative stress in human skin in vivo. J Invest Dermatol. 2004;122:1020-1029.
Wondrak GT, Jacobson MK, Jacobson EL. Endogenous UVA photosensitizers: mediators of skin photodamage and novel targets for skin photoprotection. Photochem Photobiol Sci. 2006;5:215-237.
Honigsmann H. Erythema and pigmentation. Photodermatol Photoimmunol Photomed. 2002;18:75-81.
Murakami M, Taniguchi M, Takama M, Cui JH, Oyanagui Y. UVB-dependent generation of reactive oxygen species by catalase and IgG under UVB light: Inhibition by antioxidants and anti-inflammatory drugs. Drug Discov Ther. 2008;2:85-93.
Rhie G, Shin MH, Seo JY, et al. Aging- and photoaging-dependent changes of enzymic and nonenzymic antioxidants in the epidermis and dermis of human skin in vivo. J Invest Dermatol. 2001;117:1212-1217.
Lin JY, Selim MA, Shea CR, et al. UV photoprotection by combination topical antioxidants vitamin C and vitamin E. J Am Acad Dermatol. 2003;48:866-874.
Lens M, Podesta Marty MH. Assessment of the kinetics of the antioxidative capacity of topical antioxidants. J Drugs Dermatol. 2011;10(3):262-267.
Kato S, Aoshima H, Saitoh Y, Miwa N. Biological safety of LipoFullerene composed of squalane and fullerene-C60 upon mutagenesis, photocytotoxicity, and permeability into the human skin tissue. Basic Clin Pharmacol Toxicol. 2009;104(6):483-487.