Identification of differential DNA methylation associated with multiple sclerosis: A family-based study.
Autoimmune diseases
Epigenetics
Familial multiple sclerosis
Genomics
Methylation
Multiple sclerosis
Journal
Journal of neuroimmunology
ISSN: 1872-8421
Titre abrégé: J Neuroimmunol
Pays: Netherlands
ID NLM: 8109498
Informations de publication
Date de publication:
15 07 2021
15 07 2021
Historique:
received:
09
02
2021
revised:
20
04
2021
accepted:
27
04
2021
pubmed:
16
5
2021
medline:
8
9
2021
entrez:
15
5
2021
Statut:
ppublish
Résumé
Multiple Sclerosis (MS) is caused by a still unknown interplay between genetic and environmental factors. Epigenetics, including DNA methylation, represents a model for environmental factors to influence MS risk. Twenty-six affected and 26 unaffected relatives from 8 MS multiplex families were analysed in a multicentric Italian study using MeDIP-Seq, followed by technical validation and biological replication in two additional families of differentially methylated regions (DMRs) using SeqCap Epi Choice Enrichment kit (Roche®). Associations from MeDIP-Seq across families were combined with aggregation statistics, yielding 162 DMRs at FDR ≤ 0.1. Technical validation and biological replication led to 2 hypo-methylated regions, which point to NTM and BAI3 genes, and to 2 hyper-methylated regions in PIK3R1 and CAPN13. These 4 novel regions contain genes of potential interest that need to be tested in larger cohorts of patients.
Identifiants
pubmed: 33991750
pii: S0165-5728(21)00127-2
doi: 10.1016/j.jneuroim.2021.577600
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
577600Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.