Characterization of phytochemical components and identification of main antioxidants in Crateva unilocalaris Buch. shoots by UHPLC-Q-Orbitrap-MS
ABTS•+–UHPLC–HRMS
Antioxidants
DPPH•–UHPLC–HRMS
Phenolic compounds
ROS
Saponins
Journal
Food research international (Ottawa, Ont.)
ISSN: 1873-7145
Titre abrégé: Food Res Int
Pays: Canada
ID NLM: 9210143
Informations de publication
Date de publication:
05 2021
05 2021
Historique:
received:
17
09
2020
revised:
19
02
2021
accepted:
21
02
2021
entrez:
16
5
2021
pubmed:
17
5
2021
medline:
30
6
2021
Statut:
ppublish
Résumé
The chemical constituents and antioxidant activity of the three different extracts (80% methanol, 80% ethanol, and 80% acetone) of Crateva unilocalaris Buch. shoots were investigated. Six phenolic compounds and seven saponins were characterized in all extracts. Chikusetsusaponin IVa had the highest content (17.92 to 29.16 mg/g), and chlorogenic acid was the most abundant phenolic compound (10.48 to 13.99 mg/g). The acetone extract had the highest total phenolic, flavonoid, and saponin contents and the strongest antioxidant activity. Moreover, all extracts exhibited good effects on the inhibition of intracellular ROS generation in HepG2 cells. Phenolic compounds but not saponins contributed significantly to the DPPH or ABTS radical scavenging activity of C. unilocalaris shoots according to the results of DPPH•-UHPLC-HRMS and ABTS•+-UHPLC-HRMS analyses. These results may be helpful for further understandings and utilization of C. unilocalaris shoots as a potential natural source in the food or nutraceuticals industry.
Identifiants
pubmed: 33992365
pii: S0963-9969(21)00163-0
doi: 10.1016/j.foodres.2021.110264
pii:
doi:
Substances chimiques
Antioxidants
0
Phytochemicals
0
Plant Extracts
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
110264Informations de copyright
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