Structural characterization and Kemp eliminase activity of the Mycobacterium smegmatis Ketosteroid Isomerase.

Bacterial Proteins / chemistry Biocatalysis Crystallography, X-Ray Models, Molecular Mycobacterium smegmatis / enzymology Protein Subunits / chemistry Steroid Isomerases / chemistry
Crystal structure Kemp elimination MsKSI Mycobacterium smegmatis

Journal

Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516

Informations de publication

Date de publication:
30 06 2021
Historique:
received: 03 05 2021
accepted: 04 05 2021
pubmed: 17 5 2021
medline: 27 8 2021
entrez: 16 5 2021
Statut: ppublish

Résumé

The Kemp elimination reaction, involving the ring-opening of benzoxazole and its derivatives under the action of natural enzymes or chemical catalysts, has been of interest to researchers since its discovery. Because this reaction does not exist in all currently known metabolic pathways, the computational design of Kemp eliminases has provided valuable insights into principles of enzymatic catalysis. However, it was discovered that the naturally occurring promiscuous enzymes ydbC, xapA and ketosteroid isomerase also can catalyze Kemp elimination. Here, we report the crystal structure of ketosteroid isomerase (KSI) from Mycobacterium smegmatis MC2 155. MsKSI crystallizes in the P2

Identifiants

DOI: 10.1016/j.bbrc.2021.05.007 PMID: 33992958
pubmed: 33992958
pii: S0006-291X(21)00776-2
doi: 10.1016/j.bbrc.2021.05.007
pii:
doi:

Substances chimiques

Bacterial Proteins 0
Protein Subunits 0
Steroid Isomerases EC 5.3.3.-

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

159-164

Informations de copyright

Copyright © 2021 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Yakun Liang (Y)

State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Science and College of Life Sciences, Nankai University, Tianjin, 300071, China.

Weiping Li (W)

State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Science and College of Life Sciences, Nankai University, Tianjin, 300071, China.

Han Liang (H)

State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Science and College of Life Sciences, Nankai University, Tianjin, 300071, China.

Xiaorui Lou (X)

State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Science and College of Life Sciences, Nankai University, Tianjin, 300071, China.

Ruihua Liu (R)

State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Science and College of Life Sciences, Nankai University, Tianjin, 300071, China. Electronic address: yangyangliu@nankai.edu.cn.

Qionglin Zhang (Q)

State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Science and College of Life Sciences, Nankai University, Tianjin, 300071, China. Electronic address: zql@nankai.edu.cn.

Mark Bartlam (M)

State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Science and College of Life Sciences, Nankai University, Tianjin, 300071, China.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Structural characterization and Kemp eliminase...
questionsmedicales.fr › Métabolisme › Biocatalyse › Structural characterization and Kemp eliminase...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Cristallographie aux rayons X › Structural characterization and Kemp eliminase...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles moléculaires › Structural characterization and Kemp eliminase...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets asporogènes à Gram positif › Bâtonnets asporogènes réguliers à Gram positif › Mycobacteriaceae › Mycobacterium › Mycobactéries non tuberculeuses › Mycobacterium smegmatis › Structural characterization and Kemp eliminase...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Sous-unités de protéines › Structural characterization and Kemp eliminase...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Isomerases › Intramolecular oxidoreductases › Carbon-carbon double bond isomerases › Steroid isomerases › Structural characterization and Kemp eliminase...