Mir-34c affects the proliferation and pluripotency of porcine induced pluripotent stem cell (piPSC)-like cells by targeting c-Myc.
Cell proliferation
Pluripotency
c-Myc
miR-34c
piPSC-like cells
Journal
Cells & development
ISSN: 2667-2901
Titre abrégé: Cells Dev
Pays: Netherlands
ID NLM: 101775611
Informations de publication
Date de publication:
06 2021
06 2021
Historique:
received:
17
10
2020
revised:
30
01
2021
accepted:
09
02
2021
pubmed:
18
5
2021
medline:
15
12
2021
entrez:
17
5
2021
Statut:
ppublish
Résumé
MicroRNAs are important regulators in stem cells, which involve in gene regulation, including cell proliferation, differentiation and apoptosis. As an important one, miR-34c participates in various processes by targeting protein-coding genes. It is generally considered as a tumor suppressor and cell adhesion inhibitor. However, whether miR-34c has effects on pluripotent stem cells is not clear. Here, by mir-34c mimics transfection, the function of miR-34c on porcine induced pluripotent stem cell (piPSC)-like cells was investigated. Bioinformatics analyses showed that c-Myc is miR-34c's candidate target, which was confirmed by dual Luciferase assay. The knockout of miR-34c indicated that mir-34c affects the proliferation and pluripotency of piPSC-like cells by targeting c-Myc. Our study explored the regulatory mechanism of miR-34c on piPSC-like cells, providing a reference for the establishment of true porcine PSCs.
Identifiants
pubmed: 33994350
pii: S2667-2901(21)00012-7
doi: 10.1016/j.cdev.2021.203665
pii:
doi:
Substances chimiques
MicroRNAs
0
Proto-Oncogene Proteins c-myc
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
203665Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.