Novel Tools and Investigative Approaches for the Study of Oligodendrocyte Precursor Cells (NG2-Glia) in CNS Development and Disease.
NG2-glia
genetic alteration
heterogeneity
imaging
oligodendrocyte precursor cells
sequencing
Journal
Frontiers in cellular neuroscience
ISSN: 1662-5102
Titre abrégé: Front Cell Neurosci
Pays: Switzerland
ID NLM: 101477935
Informations de publication
Date de publication:
2021
2021
Historique:
received:
26
02
2021
accepted:
07
04
2021
entrez:
17
5
2021
pubmed:
18
5
2021
medline:
18
5
2021
Statut:
epublish
Résumé
Oligodendrocyte progenitor cells (OPCs), also referred to as NG2-glia, are the most proliferative cell type in the adult central nervous system. While the primary role of OPCs is to serve as progenitors for oligodendrocytes, in recent years, it has become increasingly clear that OPCs fulfil a number of other functions. Indeed, independent of their role as stem cells, it is evident that OPCs can regulate the metabolic environment, directly interact with and modulate neuronal function, maintain the blood brain barrier (BBB) and regulate inflammation. In this review article, we discuss the state-of-the-art tools and investigative approaches being used to characterize the biology and function of OPCs. From functional genetic investigation to single cell sequencing and from lineage tracing to functional imaging, we discuss the important discoveries uncovered by these techniques, such as functional and spatial OPC heterogeneity, novel OPC marker genes, the interaction of OPCs with other cells types, and how OPCs integrate and respond to signals from neighboring cells. Finally, we review the use of
Identifiants
pubmed: 33994951
doi: 10.3389/fncel.2021.673132
pmc: PMC8116629
doi:
Types de publication
Journal Article
Langues
eng
Pagination
673132Informations de copyright
Copyright © 2021 Galichet, Clayton and Lovell-Badge.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
PLoS One. 2013 Oct 29;8(10):e78236
pubmed: 24205170
J Neurosci. 2009 Aug 12;29(32):10047-62
pubmed: 19675238
Glia. 2017 Nov;65(11):1821-1832
pubmed: 28795438
Proc Natl Acad Sci U S A. 2014 Oct 7;111(40):E4214-23
pubmed: 25246577
Development. 2011 Feb;138(4):745-53
pubmed: 21266410
Curr Protoc Cytom. 2015 Jan 05;71:12.37.1-12.37.15
pubmed: 25559221
Front Neuroanat. 2018 Oct 30;12:90
pubmed: 30425626
Methods Mol Biol. 2018;1791:131-144
pubmed: 30006706
Front Cell Neurosci. 2020 Jul 24;14:222
pubmed: 32848617
Proc Natl Acad Sci U S A. 2018 Aug 28;115(35):E8246-E8255
pubmed: 30108144
Cell Res. 2008 Jan;18(1):17-26
pubmed: 18087290
Cells. 2020 Apr 14;9(4):
pubmed: 32295179
Neurosci Lett. 2015 Jun 15;597:164-9
pubmed: 25936593
PLoS One. 2017 Feb 13;12(2):e0171947
pubmed: 28192470
J Neurosci. 2013 Sep 4;33(36):14558-66
pubmed: 24005306
Magn Reson Med. 2015 Jan;73(1):70-81
pubmed: 24604728
Science. 2016 Jul 1;353(6294):78-82
pubmed: 27365449
Glia. 2019 Feb;67(2):263-276
pubmed: 30511355
J Dev Biol. 2016 Sep 08;4(3):
pubmed: 29615592
Nat Methods. 2011 Sep 25;8(11):957-62
pubmed: 21946668
Glia. 2020 Aug;68(8):1619-1630
pubmed: 32154951
Nat Neurosci. 2008 Dec;11(12):1392-401
pubmed: 18849983
Front Genet. 2020 Sep 18;11:578712
pubmed: 33193700
J Neurosci. 2014 Sep 3;34(36):11929-47
pubmed: 25186741
FASEB J. 2008 Aug;22(8):2957-69
pubmed: 18467596
Brain Plast. 2016 Dec 21;2(1):3-29
pubmed: 29765846
J Neurosci Res. 1999 Dec 15;58(6):735-51
pubmed: 10583906
Elife. 2019 Jan 23;8:
pubmed: 30672734
Science. 2015 Mar 6;347(6226):1138-42
pubmed: 25700174
Cold Spring Harb Perspect Biol. 2015 Aug 20;8(2):a020453
pubmed: 26492571
Stem Cell Reports. 2017 Jun 6;8(6):1770-1783
pubmed: 28552605
J Neurosci. 2015 Jan 7;35(1):4-20
pubmed: 25568099
Nat Methods. 2019 Jan;16(1):33-41
pubmed: 30573813
Cell. 2007 Dec 14;131(6):1047-58
pubmed: 18083096
Nat Neurosci. 2020 Mar;23(3):456-467
pubmed: 32066983
Proteomics. 2019 Jul;19(14):e1900057
pubmed: 31216117
J Biophotonics. 2018 Feb;11(2):
pubmed: 29024450
Proc Natl Acad Sci U S A. 2019 Apr 30;116(18):9030-9039
pubmed: 30910981
Sci Rep. 2019 Jun 21;9(1):9013
pubmed: 31227736
PLoS One. 2020 Jun 8;15(6):e0233980
pubmed: 32511247
PLoS One. 2013 Nov 26;8(11):e81620
pubmed: 24303061
Exp Neurol. 2016 Sep;283(Pt B):452-75
pubmed: 27068622
Nat Neurosci. 2006 Feb;9(2):173-9
pubmed: 16388308
Exp Cell Res. 2016 Dec 10;349(2):199-213
pubmed: 27515001
Eur J Neurosci. 2007 Jan;25(2):417-24
pubmed: 17284182
Am J Hum Genet. 2017 Apr 6;100(4):617-634
pubmed: 28366443
J Neuroinflammation. 2020 Mar 19;17(1):88
pubmed: 32192522
Cell Metab. 2016 May 10;23(5):797-810
pubmed: 27166944
iScience. 2018 Aug 31;6:92-101
pubmed: 30240628
Sci Rep. 2018 Mar 5;8(1):3979
pubmed: 29507421
J Neurosci. 2018 Feb 7;38(6):1366-1382
pubmed: 29279310
Brain Struct Funct. 2017 Mar;222(2):831-847
pubmed: 27306788
PLoS Biol. 2014 Nov 11;12(11):e1001993
pubmed: 25387269
J Neurosci. 2017 Oct 18;37(42):10038-10051
pubmed: 28899915
J Struct Biol. 2013 Nov;184(2):355-60
pubmed: 24121039
Glia. 2020 Feb;68(2):345-355
pubmed: 31518022
Neurochem Res. 2020 Mar;45(3):630-642
pubmed: 31997102
Methods Mol Biol. 2013;1064:315-36
pubmed: 23996268
Trends Neurosci. 2019 Apr;42(4):263-277
pubmed: 30770136
Nat Protoc. 2020 Nov;15(11):3716-3744
pubmed: 33097924
Neuron. 2019 Feb 6;101(3):459-471.e5
pubmed: 30654924
Nat Commun. 2021 Jan 28;12(1):652
pubmed: 33510160
Cell Calcium. 2016 Nov;60(5):322-330
pubmed: 27417499
Cell Rep. 2018 Oct 23;25(4):852-861.e7
pubmed: 30355492
Front Neural Circuits. 2020 Apr 24;14:18
pubmed: 32390806
Nat Neurosci. 2019 Jul;22(7):1089-1098
pubmed: 31235908
Nat Rev Neurosci. 2015 Dec;16(12):756-67
pubmed: 26585800
Exp Mol Med. 2020 Sep;52(9):1419-1427
pubmed: 32929221
J Neurochem. 2019 Sep;150(6):691-708
pubmed: 31165473
Sci Rep. 2017 Feb 14;7:42041
pubmed: 28195192
Cancer Cell Int. 2019 Jan 14;19:16
pubmed: 30651721
Glia. 2015 Apr;63(4):699-717
pubmed: 25530205
Nat Neurosci. 2020 Dec;23(12):1496-1508
pubmed: 33139941
Neuron. 2015 Dec 2;88(5):941-956
pubmed: 26606998
Cell. 2020 Jan 9;180(1):188-204.e22
pubmed: 31883794
Neuron Glia Biol. 2008 Feb;4(1):19-26
pubmed: 19006598
Mol Cell Neurosci. 1997;9(5-6):420-32
pubmed: 9361279
Stem Cell Reports. 2017 Apr 11;8(4):803-812
pubmed: 28344001
Cell Stem Cell. 2013 Feb 7;12(2):252-64
pubmed: 23395447
Front Cell Neurosci. 2020 Oct 16;14:558381
pubmed: 33177991
J Neurosci. 2008 Jan 2;28(1):264-78
pubmed: 18171944
Neuron. 2010 Nov 18;68(4):668-81
pubmed: 21092857
Science. 2016 Aug 26;353(6302):925-8
pubmed: 27471252
FEBS Lett. 2018 Apr;592(7):1063-1078
pubmed: 29427507
Dev Cell. 2018 Aug 20;46(4):504-517.e7
pubmed: 30078729
Biochemistry. 1980 May 27;19(11):2396-404
pubmed: 6770893
Neuropharmacology. 2016 Nov;110(Pt B):563-573
pubmed: 26282119
Neuron. 2006 Jul 20;51(2):187-99
pubmed: 16846854
J Neurosci Res. 2009 Nov 15;87(15):3390-402
pubmed: 19360905
Mol Cell Neurosci. 2003 Oct;24(2):476-88
pubmed: 14572468
Biochim Biophys Acta. 2014 Sep;1843(9):1917-29
pubmed: 24768715
Front Cell Neurosci. 2016 Aug 22;10:203
pubmed: 27597818
Magn Reson Med Sci. 2016;15(1):11-25
pubmed: 26346405
Nat Biotechnol. 2021 Apr;39(4):510-519
pubmed: 33257861
Methods Mol Biol. 2019;1936:275-294
pubmed: 30820905
J Biol Chem. 1985 Mar 25;260(6):3440-50
pubmed: 3838314
J Neurosci. 2009 May 20;29(20):6663-76
pubmed: 19458236
Development. 2008 Jan;135(1):145-57
pubmed: 18045844
J Neurosci. 2016 Oct 19;36(42):10853-10869
pubmed: 27798140
Cereb Cortex. 2020 May 18;30(6):3800-3819
pubmed: 31989178
Dev Neurosci. 2001;23(4-5):346-55
pubmed: 11756750
Brain Circ. 2016 Jul-Sep;2(3):121-125
pubmed: 30276286
J Neuroinflammation. 2020 May 6;17(1):146
pubmed: 32375817
Sci Rep. 2018 Feb 1;8(1):2113
pubmed: 29391408
PLoS One. 2014 Jul 31;9(7):e103174
pubmed: 25078775
J Vis Exp. 2020 Nov 9;(165):
pubmed: 33226027
Glia. 2020 Jun;68(6):1291-1303
pubmed: 31958186
Cell Rep. 2018 Apr 24;23(4):1220-1229
pubmed: 29694897
Dev Dyn. 2001 Oct;222(2):218-27
pubmed: 11668599
Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2243-E2252
pubmed: 28246330
Biochim Biophys Acta. 2012 Sep;1823(9):1406-15
pubmed: 22521452
Cells. 2020 Mar 03;9(3):
pubmed: 32138223
Glia. 2008 Sep;56(12):1339-52
pubmed: 18512250
Nat Neurosci. 2016 Feb;19(2):335-46
pubmed: 26727548
J Biol Chem. 2008 Jan 18;283(3):1637-1643
pubmed: 17993465
Front Neurosci. 2018 Oct 26;12:733
pubmed: 30416422
Mol Cell Neurosci. 2002 May;20(1):30-42
pubmed: 12056838
BMC Res Notes. 2016 Sep 05;9(1):419
pubmed: 27592856
Nat Neurosci. 2020 Mar;23(3):363-374
pubmed: 32066987
Nature. 2017 May 4;545(7652):54-59
pubmed: 28445465
Nat Methods. 2017 Oct;14(10):955-958
pubmed: 28846088
Nat Protoc. 2007;2(5):1044-51
pubmed: 17546009
J Neurosci. 2010 May 19;30(20):6921-9
pubmed: 20484634
Nature. 2000 May 11;405(6783):187-91
pubmed: 10821275
Methods. 2020 Mar 1;174:3-10
pubmed: 31326595
Assay Drug Dev Technol. 2011 Jun;9(3):281-9
pubmed: 21184646
Neurosci Lett. 2020 Jan 10;715:134593
pubmed: 31678373
Neurosci Res. 2020 Mar;152:66-77
pubmed: 31923448
Elife. 2019 Oct 18;8:
pubmed: 31625910
Front Cell Dev Biol. 2020 Apr 16;8:220
pubmed: 32373607
PLoS One. 2011 Mar 24;6(3):e17575
pubmed: 21455301
Proc IEEE Int Symp Biomed Imaging. 2018 Apr;2018:961-964
pubmed: 30598726
Front Cell Neurosci. 2018 Dec 06;12:477
pubmed: 30574070
Exp Mol Med. 2017 Jul 28;49(7):e361
pubmed: 28751784
J Comp Neurol. 2017 Feb 15;525(3):535-552
pubmed: 27448245
Neuroscience. 2020 May 1;433:132-143
pubmed: 32171821
Glia. 2014 Jun;62(6):896-913
pubmed: 24578301
Adv Exp Med Biol. 2016;949:27-45
pubmed: 27714683
Nat Protoc. 2015 Aug;10(8):1143-54
pubmed: 26134954
Trends Neurosci. 2001 Jan;24(1):39-47
pubmed: 11163886
Development. 2015 Dec 1;142(23):3983-95
pubmed: 26628089
eNeuro. 2020 Aug 17;7(4):
pubmed: 32409508
Neuroscience. 2014 Sep 12;276:148-60
pubmed: 24161723
Med Hypotheses. 2000 Apr;54(4):553-64
pubmed: 10859638
PLoS One. 2015 May 12;10(5):e0127222
pubmed: 25966014
J Interferon Cytokine Res. 2009 Jun;29(6):313-26
pubmed: 19441883
Cells. 2019 Nov 12;8(11):
pubmed: 31726662
Nat Commun. 2018 Sep 13;9(1):3708
pubmed: 30213958
Sci Rep. 2020 Mar 20;10(1):5137
pubmed: 32198485
Nature. 2014 Apr 10;508(7495):207-14
pubmed: 24695228
Cold Spring Harb Perspect Biol. 2015 Jan 29;7(5):
pubmed: 25635044
Cell Rep. 2017 Mar 28;18(13):3227-3241
pubmed: 28355573
Cell Stem Cell. 2018 Aug 02;23(2):239-251.e6
pubmed: 30075130
Glia. 2019 Jun;67(6):1094-1103
pubmed: 30724411
Exp Neurol. 2015 Mar;265:69-83
pubmed: 25542980
J Neurosci. 2018 Jul 18;38(29):6586-6596
pubmed: 29941446
Exp Mol Med. 2018 Aug 7;50(8):1-14
pubmed: 30089861
Proc Natl Acad Sci U S A. 2019 Jun 4;116(23):11480-11489
pubmed: 31101714
J Vis Exp. 2011 Aug 21;(54):
pubmed: 21876528
Biol Open. 2016 Apr 15;5(4):461-74
pubmed: 26988758
Cell Mol Neurobiol. 2019 Nov;39(8):1071-1080
pubmed: 31222426
Lab Anim. 2021 Feb 17;:23677221993141
pubmed: 33596732
Stem Cell Reports. 2019 Dec 10;13(6):1053-1067
pubmed: 31708478
Methods Cell Biol. 1989;30:127-56
pubmed: 2538708
J Am Heart Assoc. 2019 May 7;8(9):e011824
pubmed: 31020902
Anal Chim Acta. 2017 Apr 29;964:7-23
pubmed: 28351641
Neurosci Lett. 2020 Jun 11;729:134959
pubmed: 32339610
Brain Res. 2016 May 1;1638(Pt B):138-160
pubmed: 26385417
PLoS Biol. 2017 Aug 22;15(8):e2001993
pubmed: 28829781
J Neurosci. 2020 Sep 30;40(40):7609-7624
pubmed: 32868463
J Neurosci. 2010 Sep 8;30(36):12036-49
pubmed: 20826667
Pediatr Nephrol. 2011 Sep;26(9):1483-9
pubmed: 21404099
Glia. 2020 Feb;68(2):376-392
pubmed: 31605513
J Neurosci. 2012 Jun 13;32(24):8173-85
pubmed: 22699898
Front Cell Dev Biol. 2016 Jun 28;4:71
pubmed: 27551677
PLoS Biol. 2020 Apr 10;18(4):e3000665
pubmed: 32275651
Glia. 2019 Oct;67(10):1797-1805
pubmed: 30968471
Nat Neurosci. 2019 Mar;22(3):484-491
pubmed: 30692691
Science. 2016 Jun 10;352(6291):1326-1329
pubmed: 27284195
Adv Sci (Weinh). 2020 Oct 29;7(23):2002155
pubmed: 33304759
Nat Methods. 2018 Sep;15(9):700-706
pubmed: 30046099
Glia. 2020 Apr;68(4):705-720
pubmed: 31633852
Glia. 2015 Aug;63(8):1429-51
pubmed: 26010717
Glia. 2020 Sep;68(9):1692-1728
pubmed: 31958188
Front Cell Neurosci. 2017 Sep 14;11:284
pubmed: 28959189
Stem Cell Res. 2019 May;37:101429
pubmed: 30933718
Cells. 2020 Jul 29;9(8):
pubmed: 32751289
BMC Med. 2019 Nov 15;17(1):204
pubmed: 31727112
Mol Cell Neurosci. 2004 Feb;25(2):252-62
pubmed: 15019942
Biochem Biophys Res Commun. 2007 Jan 19;352(3):598-602
pubmed: 17150193
Pharmaceut Med. 2011 Oct 1;25(5):293-306
pubmed: 22200988
J Neurosci. 2007 Mar 28;27(13):3416-28
pubmed: 17392458
Development. 2009 May;136(9):1443-52
pubmed: 19363151
Stem Cell Reports. 2016 Jan 12;6(1):1-8
pubmed: 26724902
J Neurosci Res. 2000 Sep 1;61(5):471-9
pubmed: 10956416
eNeuro. 2017 Oct 16;4(5):
pubmed: 29085899
Nat Neurosci. 2004 Jan;7(1):24-32
pubmed: 14661022
Glia. 2002 Mar 15;37(4):307-13
pubmed: 11870870
J Clin Invest. 2013 Feb;123(2):782-6
pubmed: 23281396