Analysis of appendectomy samples identified dysbiosis in acute appendicitis.
appendectomy
appendicitis
dysbiosis
Journal
Bioscience of microbiota, food and health
ISSN: 2186-6953
Titre abrégé: Biosci Microbiota Food Health
Pays: Japan
ID NLM: 101578264
Informations de publication
Date de publication:
2021
2021
Historique:
received:
10
08
2020
accepted:
24
10
2020
entrez:
17
5
2021
pubmed:
18
5
2021
medline:
18
5
2021
Statut:
ppublish
Résumé
Appendicitis is the most common cause of sudden-onset abdominal pain requiring surgery. Culture-independent techniques have revealed that the complex intestinal bacterial ecology is associated with various diseases. To evaluate differences in patient characteristics and gut microbiota distribution in patients with appendicitis, we enrolled 12 patients who underwent appendectomy for appendicitis (appendicitis group) and 13 patients who underwent ileocecal resection or right hemicolectomy for colon cancer (control group). Microbiota were analyzed using next-generation sequencing of surgical specimens from appendix swab samples collected postoperatively. Overall differences in the structure of the gut microbiota were evaluated using the α- and β-diversity indices, which were calculated using the weighted or unweighted UniFrac distance. Changes in the gut microbial distribution were taxonomically evaluated at the phylum and genus levels. The α-diversity of observed species was significantly different between patients with and without inflammation of the appendix. The appendiceal microbiome of patients with appendicitis exhibited the highest unweighted UniFrac distances. There were no significant differences at the phylum level.
Identifiants
pubmed: 33996365
doi: 10.12938/bmfh.2020-051
pii: 2020-051
pmc: PMC8099629
doi:
Types de publication
Journal Article
Langues
eng
Pagination
92-97Informations de copyright
©2021 BMFH Press.
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