Comparing periodontitis biomarkers in saliva, oral rinse and gingival crevicular fluid: A pilot study.
MMP-8
biomarkers
chitinase
gingival crevicular fluid
modelling
oral rinse
periodontitis
principal component analysis (PCA)
protease
saliva
Journal
Journal of clinical periodontology
ISSN: 1600-051X
Titre abrégé: J Clin Periodontol
Pays: United States
ID NLM: 0425123
Informations de publication
Date de publication:
09 2021
09 2021
Historique:
revised:
24
03
2021
received:
03
09
2020
accepted:
15
04
2021
pubmed:
18
5
2021
medline:
1
6
2022
entrez:
17
5
2021
Statut:
ppublish
Résumé
To explore the feasibility of screening for periodontitis by measuring biomarkers, namely total proteolytic activity (TPA), matrix metalloproteinase (MMP)-8, chitinase, lysozyme or their combination, in saliva, oral rinse and gingival crevicular fluid (GCF). Subjects were recruited among healthy/gingivitis individuals and untreated periodontitis patients in Academic Centre for Dentistry Amsterdam (ACTA). All participants donated samples of unstimulated whole saliva, oral rinse and GCF. The protein concentrations and MMP-8 levels were determined by ELISA. Enzymatic activities were measured using appropriate fluorogenic substrates. In oral rinse samples, periodontitis patients (n = 19) exhibited significantly higher concentrations of MMP-8 and TPA than controls (n = 20). MMP-8 in combination with chitinase explained 88% of the variance and assigned a subject to control or periodontitis group, with best accuracy (87.2%) in oral rinse. The combination of MMP-8 and chitinase in the current oral rinse procedure has the potential to discriminate periodontitis from periodontal health/gingivitis.
Identifiants
pubmed: 33998029
doi: 10.1111/jcpe.13479
pmc: PMC8453974
doi:
Substances chimiques
Biomarkers
0
Matrix Metalloproteinase 8
EC 3.4.24.34
Types de publication
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1250-1259Informations de copyright
© 2021 The Authors. Journal of Clinical Periodontology published by John Wiley & Sons Ltd.
Références
J Periodontal Res. 2015 Jun;50(3):330-6
pubmed: 25040400
J Periodontal Res. 2002 Aug;37(4):245-9
pubmed: 12200966
J Clin Periodontol. 2021 Sep;48(9):1250-1259
pubmed: 33998029
J Clin Periodontol. 2018 Dec;45(12):1421-1428
pubmed: 30341955
Inflammation. 2014 Oct;37(5):1771-8
pubmed: 24799321
Mol Diagn Ther. 2019 Oct;23(5):667-676
pubmed: 31372941
J Clin Periodontol. 1986 Jan;13(1):19-26
pubmed: 2418067
Scand J Haematol. 1979 Feb;22(2):173-8
pubmed: 432552
Cent Eur J Immunol. 2014;39(3):357-64
pubmed: 26155148
Diagnostics (Basel). 2019 Mar 23;9(1):
pubmed: 30909530
Eur J Clin Invest. 2016 Sep;46(9):757-65
pubmed: 27296149
BMC Oral Health. 2019 May 23;19(1):87
pubmed: 31122214
Oral Dis. 2004 Nov;10(6):311-8
pubmed: 15533204
J Periodontal Res. 1995 Jan;30(1):73-9
pubmed: 7722849
J Dent Res. 2014 Nov;93(11):1045-53
pubmed: 25261053
Eur J Oral Sci. 2015 Jun;123(3):149-57
pubmed: 25809904
Pharmacol Res. 2011 Feb;63(2):108-13
pubmed: 20937384
Periodontol 2000. 2003;31:77-104
pubmed: 12656997
Eur J Oral Sci. 1999 Oct;107(5):328-37
pubmed: 10515197
Periodontol 2000. 2016 Feb;70(1):65-79
pubmed: 26662483
J Am Dent Assoc. 1994 Feb;125(2):163-9; discussion 169-71
pubmed: 8113524
J Clin Periodontol. 2005;32 Suppl 6:210-3
pubmed: 16128839
Scand J Clin Lab Invest Suppl. 1994;219:47-54
pubmed: 7701241
Int J Oral Sci. 2016 Sep 29;8(3):191-8
pubmed: 27515277
J Periodontal Res. 2003 Aug;38(4):436-9
pubmed: 12828663
Periodontol 2000. 2016 Feb;70(1):53-64
pubmed: 26662482
Periodontol 2000. 2016 Feb;70(1):142-63
pubmed: 26662488
J Periodontol. 2018 Jun;89 Suppl 1:S173-S182
pubmed: 29926951
Acta Medica (Hradec Kralove). 2016;59(1):3-9
pubmed: 27131349
J Clin Periodontol. 2020 Jan;47(1):2-18
pubmed: 31560804
Proteomics Clin Appl. 2014 Oct;8(9-10):748-61
pubmed: 24828325
Diagnostics (Basel). 2018 Oct 22;8(4):
pubmed: 30360358
Periodontol 2000. 2003;31:9-11
pubmed: 12656992
J Periodontol. 2016 Jan;87(1):36-40
pubmed: 26430926
Glycobiology. 1994 Dec;4(6):797-803
pubmed: 7734843
J Clin Periodontol. 2011 Sep;38(9):817-9
pubmed: 21707693
Oral Dis. 2018 Apr;24(3):363-371
pubmed: 28922514
J Clin Periodontol. 2018 Sep;45(9):1016-1024
pubmed: 29971805
Eur J Intern Med. 2015 Sep;26(7):498-503
pubmed: 26160771
Med Sci Monit. 2009 Feb;15(2):CR66-73
pubmed: 19179970
Arch Oral Biol. 1992 Jan;37(1):23-8
pubmed: 1596205
Infect Immun. 1995 Dec;63(12):4770-3
pubmed: 7591134