Impairment of the ER/mitochondria compartment in human cardiomyocytes with PLN p.Arg14del mutation.


Journal

EMBO molecular medicine
ISSN: 1757-4684
Titre abrégé: EMBO Mol Med
Pays: England
ID NLM: 101487380

Informations de publication

Date de publication:
07 06 2021
Historique:
revised: 31 03 2021
received: 07 07 2020
accepted: 07 04 2021
pubmed: 18 5 2021
medline: 26 10 2021
entrez: 17 5 2021
Statut: ppublish

Résumé

The phospholamban (PLN) p.Arg14del mutation causes dilated cardiomyopathy, with the molecular disease mechanisms incompletely understood. Patient dermal fibroblasts were reprogrammed to hiPSC, isogenic controls were established by CRISPR/Cas9, and cardiomyocytes were differentiated. Mutant cardiomyocytes revealed significantly prolonged Ca

Identifiants

pubmed: 33998164
doi: 10.15252/emmm.202013074
pmc: PMC8185541
doi:

Substances chimiques

Calcium-Binding Proteins 0
phospholamban 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e13074

Subventions

Organisme : National Centre for the Replacement, Refinement and Reduction of Animals in Research
ID : NC/T2T0119
Pays : United Kingdom
Organisme : British Heart Foundation
ID : RM/13/30157
Pays : United Kingdom
Organisme : British Heart Foundation
ID : CH/16/3/32406
Pays : United Kingdom
Organisme : National Centre for the Replacement, Refinement and Reduction of Animals in Research
ID : NC/S001808/1
Pays : United Kingdom
Organisme : British Heart Foundation
ID : RG/14/1/30588
Pays : United Kingdom
Organisme : National Centre for the Replacement, Refinement and Reduction of Animals in Research
ID : NC/C013202/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/L012618/1
Pays : United Kingdom
Organisme : National Centre for the Replacement, Refinement and Reduction of Animals in Research
ID : NC/C013105/1
Pays : United Kingdom
Organisme : British Heart Foundation
ID : RG/16/14/32397
Pays : United Kingdom

Informations de copyright

© 2021 The Authors. Published under the terms of the CC BY 4.0 license.

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Auteurs

Friederike Cuello (F)

Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
German Center for Heart Research (DZHK), Kiel, Germany.

Anika E Knaust (AE)

Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
German Center for Heart Research (DZHK), Kiel, Germany.

Umber Saleem (U)

Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
German Center for Heart Research (DZHK), Kiel, Germany.

Malte Loos (M)

Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
German Center for Heart Research (DZHK), Kiel, Germany.

Janice Raabe (J)

Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
German Center for Heart Research (DZHK), Kiel, Germany.

Diogo Mosqueira (D)

Division of Cancer & Stem Cells, Biodiscovery Institute, University of Nottingham, Nottingham, UK.

Sandra Laufer (S)

Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
German Center for Heart Research (DZHK), Kiel, Germany.

Michaela Schweizer (M)

Electron Microscopy Unit, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Petra van der Kraak (P)

Department of Pathology, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands.

Frederik Flenner (F)

Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
German Center for Heart Research (DZHK), Kiel, Germany.

Bärbel M Ulmer (BM)

Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
German Center for Heart Research (DZHK), Kiel, Germany.

Ingke Braren (I)

German Center for Heart Research (DZHK), Kiel, Germany.
Vector Core Unit, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Xiaoke Yin (X)

King's British Heart Foundation Centre of Research Excellence, King's College London, London, UK.

Konstantinos Theofilatos (K)

King's British Heart Foundation Centre of Research Excellence, King's College London, London, UK.

Jorge Ruiz-Orera (J)

Cardiovascular and Metabolic Sciences, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.

Giannino Patone (G)

Cardiovascular and Metabolic Sciences, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.

Birgit Klampe (B)

Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
German Center for Heart Research (DZHK), Kiel, Germany.

Thomas Schulze (T)

Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
German Center for Heart Research (DZHK), Kiel, Germany.

Angelika Piasecki (A)

Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
German Center for Heart Research (DZHK), Kiel, Germany.

Yigal Pinto (Y)

Department of Experimental Cardiology, Academic Medical Center, Amsterdam, The Netherlands.

Aryan Vink (A)

Department of Pathology, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands.

Norbert Hübner (N)

Cardiovascular and Metabolic Sciences, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.
DZHK (German Centre for Cardiovascular Research), Berlin, Germany.
Charité -Universitätsmedizin, Berlin, Germany.
Berlin Institute of Health (BIH), Berlin, Germany.

Sian Harding (S)

British Heart Foundation Centre of Research Excellence, NHLI, Imperial College London, London, UK.

Manuel Mayr (M)

King's British Heart Foundation Centre of Research Excellence, King's College London, London, UK.

Chris Denning (C)

Division of Cancer & Stem Cells, Biodiscovery Institute, University of Nottingham, Nottingham, UK.

Thomas Eschenhagen (T)

Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
German Center for Heart Research (DZHK), Kiel, Germany.

Arne Hansen (A)

Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
German Center for Heart Research (DZHK), Kiel, Germany.

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