The needle and the haystack: single molecule tracking to probe the transcription factor search in eukaryotes.
search mechanism
single molecule
transcription factors
Journal
Biochemical Society transactions
ISSN: 1470-8752
Titre abrégé: Biochem Soc Trans
Pays: England
ID NLM: 7506897
Informations de publication
Date de publication:
30 06 2021
30 06 2021
Historique:
received:
11
03
2021
revised:
18
04
2021
accepted:
19
04
2021
pubmed:
19
5
2021
medline:
1
2
2022
entrez:
18
5
2021
Statut:
ppublish
Résumé
Transcription factors (TFs) regulate transcription of their target genes by identifying and binding to regulatory regions of the genome among billions of potential non-specific decoy sites, a task that is often presented as a 'needle in the haystack' challenge. The TF search process is now well understood in bacteria, but its characterization in eukaryotes needs to account for the complex organization of the nuclear environment. Here we review how live-cell single molecule tracking is starting to shed light on the TF search mechanism in the eukaryotic cell and we outline the future challenges to tackle in order to understand how nuclear organization modulates the TF search process in physiological and pathological conditions.
Identifiants
pubmed: 34003257
pii: 228690
doi: 10.1042/BST20200709
pmc: PMC8286828
doi:
Substances chimiques
Transcription Factors
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
1121-1132Informations de copyright
© 2021 The Author(s).
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