The rescue and selection of thermally stable type O vaccine candidate strains of foot-and-mouth disease virus.

Amino Acid Substitution Animals Cell Line Cricetinae Drug Storage Foot-and-Mouth Disease / prevention & control Foot-and-Mouth Disease Virus / genetics Guinea Pigs Immunization Neutralization Tests Poverty Serogroup Swine Vaccines, Inactivated / administration & dosage Viral Structural Proteins / genetics Viral Vaccines / administration & dosage Virus Replication / drug effects

Journal

Archives of virology
ISSN: 1432-8798
Titre abrégé: Arch Virol
Pays: Austria
ID NLM: 7506870

Informations de publication

Date de publication:
Aug 2021
Historique:
received: 23 09 2020
accepted: 26 03 2021
pubmed: 19 5 2021
medline: 17 7 2021
entrez: 18 5 2021
Statut: ppublish

Résumé

Inactivated foot-and-mouth disease virus (FMDV) vaccines have been used widely to control foot-and-mouth disease (FMD). However, the virions (146S) of this virus are easily dissociated into pentamer subunits (12S), which limits the immune protective efficacy of inactivated vaccines when the temperature is higher than 30 °C. A cold-chain system can maintain the quality of the vaccines, but such systems are usually not reliable in limited-resource settings. Thus, it is imperative to improve the thermostability of vaccine strains to guarantee the quality of the vaccines. In this study, four recombinant FMDV strains containing single or multiple amino acid substitutions in the structural proteins were rescued using a previously constructed FMDV type O full-length infectious clone (pO/DY-VP1). We found that single or multiple amino acid substitutions in the structural proteins affected viral replication to different degrees. Furthermore, the heat and acid stability of the recombinant viruses was significantly increased when compared with the parental virus. Three thermally stable recombinant viruses (rHN/DY-VP1

Identifiants

DOI: 10.1007/s00705-021-05100-3 PMID: 34003358
pubmed: 34003358
doi: 10.1007/s00705-021-05100-3
pii: 10.1007/s00705-021-05100-3
doi:

Substances chimiques

Vaccines, Inactivated 0
Viral Structural Proteins 0
Viral Vaccines 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

2131-2140

Informations de copyright

© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.

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Auteurs

Ya Gao (Y)

State Key Laboratory of Veterinary Etiologic Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, China.

Pinghua Li (P)

State Key Laboratory of Veterinary Etiologic Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, China.

Xueqing Ma (X)

State Key Laboratory of Veterinary Etiologic Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, China.

Xingwen Bai (X)

State Key Laboratory of Veterinary Etiologic Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, China.

Pu Sun (P)

State Key Laboratory of Veterinary Etiologic Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, China.

Ping Du (P)

State Key Laboratory of Veterinary Etiologic Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, China.

Hong Yuan (H)

State Key Laboratory of Veterinary Etiologic Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, China.

Yimei Cao (Y)

State Key Laboratory of Veterinary Etiologic Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, China.

Kun Li (K)

State Key Laboratory of Veterinary Etiologic Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, China.

Yuanfang Fu (Y)

State Key Laboratory of Veterinary Etiologic Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, China.

Jing Zhang (J)

State Key Laboratory of Veterinary Etiologic Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, China.

Huifang Bao (H)

State Key Laboratory of Veterinary Etiologic Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, China.

Yingli Chen (Y)

State Key Laboratory of Veterinary Etiologic Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, China.

Zhiyong Li (Z)

State Key Laboratory of Veterinary Etiologic Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, China.

Zengjun Lu (Z)

State Key Laboratory of Veterinary Etiologic Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, China. luzengjun@caas.cn.

Zaixin Liu (Z)

State Key Laboratory of Veterinary Etiologic Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, China. liuzaixin@caas.cn.

Dong Li (D)

State Key Laboratory of Veterinary Etiologic Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, China. lidong@caas.cn.
ORCID: 0000-0002-9101-0241

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Mutagenèse › Substitution d'acide aminé › The rescue and selection of thermally stable...
questionsmedicales.fr › Eucaryotes › Animaux › The rescue and selection of thermally stable...
questionsmedicales.fr › Cellules › Cellules cultivées › Lignée cellulaire › The rescue and selection of thermally stable...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Arvicolinae › Cricetinae › The rescue and selection of thermally stable...
questionsmedicales.fr › Techniques d'investigation › Stockage de médicament › The rescue and selection of thermally stable...
questionsmedicales.fr › Maladies de l'animal › Fièvre aphteuse › The rescue and selection of thermally stable...
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN à brin positif › Picornaviridae › Aphthovirus › Virus de la fièvre aphteuse › The rescue and selection of thermally stable...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Cochons d'Inde › The rescue and selection of thermally stable...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Pratiques en santé publique › Prévention primaire › Immunisation › The rescue and selection of thermally stable...
questionsmedicales.fr › Techniques d'investigation › Techniques immunologiques › Tests immunologiques › Tests sérologiques › Tests de neutralisation › The rescue and selection of thermally stable...
questionsmedicales.fr › Caractéristiques de la population › Facteurs socioéconomiques › Pauvreté › The rescue and selection of thermally stable...
questionsmedicales.fr › Phénomènes génétiques › Phénotype › Sérogroupe › The rescue and selection of thermally stable...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Suidae › The rescue and selection of thermally stable...
questionsmedicales.fr › Mélanges complexes › Produits biologiques › Vaccins › Vaccins inactivés › The rescue and selection of thermally stable...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Protéines virales structurales › The rescue and selection of thermally stable...
questionsmedicales.fr › Mélanges complexes › Produits biologiques › Vaccins › Vaccins antiviraux › The rescue and selection of thermally stable...
questionsmedicales.fr › Phénomènes microbiologiques › Phénomènes physiologiques viraux › Réplication virale › The rescue and selection of thermally stable...