Transcriptome-based screening of ion channels and transporters in a migratory chondroprogenitor cell line isolated from late-stage osteoarthritic cartilage.
Calcium Signaling
Cartilage, Articular
/ drug effects
Cell Line
Cell Movement
/ drug effects
Cell Proliferation
Chondrocytes
/ drug effects
Core Binding Factor Alpha 1 Subunit
/ genetics
Gene Expression Profiling
Humans
Ion Channels
/ genetics
Large-Conductance Calcium-Activated Potassium Channel alpha Subunits
/ genetics
Membrane Potentials
Membrane Transport Proteins
/ genetics
Osteoarthritis, Knee
/ genetics
Potassium Channel Blockers
/ pharmacology
Stem Cells
/ drug effects
Time Factors
Transcriptome
cartilage
channelome
chondrocyte
chondroprogenitor
mesenchymal stem cell
osteoarthritis
transcriptomics
Journal
Journal of cellular physiology
ISSN: 1097-4652
Titre abrégé: J Cell Physiol
Pays: United States
ID NLM: 0050222
Informations de publication
Date de publication:
11 2021
11 2021
Historique:
revised:
28
04
2021
received:
13
11
2020
accepted:
04
05
2021
pubmed:
20
5
2021
medline:
15
12
2021
entrez:
19
5
2021
Statut:
ppublish
Résumé
Chondrogenic progenitor cells (CPCs) may be used as an alternative source of cells with potentially superior chondrogenic potential compared to mesenchymal stem cells (MSCs), and could be exploited for future regenerative therapies targeting articular cartilage in degenerative diseases such as osteoarthritis (OA). In this study, we hypothesised that CPCs derived from OA cartilage may be characterised by a distinct channelome. First, a global transcriptomic analysis using Affymetrix microarrays was performed. We studied the profiles of those ion channels and transporter families that may be relevant to chondroprogenitor cell physiology. Following validation of the microarray data with quantitative reverse transcription-polymerase chain reaction, we examined the role of calcium-dependent potassium channels in CPCs and observed functional large-conductance calcium-activated potassium (BK) channels involved in the maintenance of the chondroprogenitor phenotype. In line with our very recent results, we found that the KCNMA1 gene was upregulated in CPCs and observed currents that could be attributed to the BK channel. The BK channel inhibitor paxilline significantly inhibited proliferation, increased the expression of the osteogenic transcription factor RUNX2, enhanced the migration parameters, and completely abolished spontaneous Ca
Substances chimiques
Core Binding Factor Alpha 1 Subunit
0
Ion Channels
0
KCNMA1 protein, human
0
Large-Conductance Calcium-Activated Potassium Channel alpha Subunits
0
Membrane Transport Proteins
0
Potassium Channel Blockers
0
RUNX2 protein, human
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
7421-7439Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/E022758/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/I001271/1
Pays : United Kingdom
Organisme : Arthritis Research UK
ID : 20194
Pays : United Kingdom
Organisme : Marie Curie
ID : 625746
Pays : United Kingdom
Informations de copyright
© 2021 The Authors. Journal of Cellular Physiology published by Wiley Periodicals LLC.
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