Recombinant Protein Production and Purification Using Eukaryotic Cell Factories.
Cloning
Fermentation
Ion exchange chromatography
Lipase
Pichia pastoris
Recombinant protein
Rhizomucor miehei
SDS-PAGE
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
19
5
2021
pubmed:
20
5
2021
medline:
23
6
2021
Statut:
ppublish
Résumé
Cloning proteins enables their production and characterization for further studies. This requires inserting the gene of the studied protein to be inserted in a vector, which then will be transformed to the host cell used as "factory." Consequently, the "biomass" of host cells will be produced using bioreactors. Here we describe the production of Rhizomucor miehei lipase (RML) by cloning the corresponding genes in the yeast Pichia pastoris. This enzyme is used as a biocatalyst for biofuel production. The successfully produced recombinant proteins are then purified using ion exchange chromatography.
Identifiants
pubmed: 34009593
doi: 10.1007/978-1-0716-1323-8_15
doi:
Substances chimiques
Recombinant Proteins
0
Lipase
EC 3.1.1.3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
215-228Références
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