Infant-Associated Bifidobacterial β-Galactosidases and Their Ability to Synthesize Galacto-Oligosaccharides.
Bifidobacterium
bifidogenic
galacto-oligosaccharides
gut microbiota
infant
microbiome
oligosaccharides
prebiotics
Journal
Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977
Informations de publication
Date de publication:
2021
2021
Historique:
received:
03
02
2021
accepted:
01
04
2021
entrez:
20
5
2021
pubmed:
21
5
2021
medline:
21
5
2021
Statut:
epublish
Résumé
Galacto-oligosaccharides (GOS) represent non-digestible glycans that are commercially produced by transgalactosylation of lactose, and that are widely used as functional food ingredients in prebiotic formulations, in particular in infant nutrition. GOS consumption has been reported to enhance growth of specific bacteria in the gut, in particular bifidobacteria, thereby supporting a balanced gut microbiota. In a previous study, we assessed the hydrolytic activity and substrate specificity of seventeen predicted β-galactosidases encoded by various species and strains of infant-associated bifidobacteria. In the current study, we further characterized seven out of these seventeen bifidobacterial β-galactosidases in terms of their kinetics, enzyme stability and oligomeric state. Accordingly, we established whether these β-galactosidases are capable of synthesizing GOS via enzymatic transgalactosylation employing lactose as the feed substrate. Our findings show that the seven selected enzymes all possess such transgalactosylation activity, though they appear to differ in their efficiency by which they perform this reaction. From chromatography analysis, it seems that these enzymes generate two distinct GOS mixtures: GOS with a relatively short or long degree of polymerization profile. These findings may be the stepping stone for further studies aimed at synthesizing new GOS variants with novel and/or enhanced prebiotic activities and potential for industrial applications.
Identifiants
pubmed: 34012427
doi: 10.3389/fmicb.2021.662959
pmc: PMC8126724
doi:
Types de publication
Journal Article
Langues
eng
Pagination
662959Informations de copyright
Copyright © 2021 Ambrogi, Bottacini, O’Callaghan, Casey, van Breen, Schoemaker, Cao, Kuipers, O’Connell Motherway, Schoterman and van Sinderen.
Déclaration de conflit d'intérêts
MS, BK, and LC are employees of FrieslandCampina. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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