Neutrophil Extracellular Traps Serve as Key Effector Molecules in the Protection Against Phialophora verrucosa.
Fungi killing
Neutrophil extracellular traps
Neutrophils
Phialophora verrucosa
Journal
Mycopathologia
ISSN: 1573-0832
Titre abrégé: Mycopathologia
Pays: Netherlands
ID NLM: 7505689
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
received:
30
12
2020
accepted:
10
04
2021
pubmed:
21
5
2021
medline:
16
9
2021
entrez:
20
5
2021
Statut:
ppublish
Résumé
Phialophora verrucosa (P. verrucosa) is a pathogen that can cause chromoblastomycosis and phaeohyphomycosis. Recent evidence suggests that neutrophils can produce neutrophil extracellular traps (NETs) that can protect against invasive pathogens. As such, we herein explored the in vitro functional importance of P. verrucosa-induced NET formation. By assessing the co-localization of neutrophil elastase and DNA, we were able to confirm the formation of classical NETs entrapping P. verrucosa specimens. Sytox Green was then used to stain these NETs following neutrophil infection with P. verrucosa in order to quantify the formation of these extracellular structures. NET formation was induced upon neutrophil exposure to both live, UV-inactivated, and dead P. verrucosa fungi. The ability of these NETs to kill fungal hyphae and conidia was demonstrated through MTT and pouring plate assays, respectively. Overall, our results confirmed that P. verrucosa was able to trigger the production of NETs, suggesting that these extracellular structures may represent an important innate immune effector mechanism controlling physiological responses to P. verrucosa infection, thereby aiding in pathogen control during the acute phases of infection.
Identifiants
pubmed: 34013384
doi: 10.1007/s11046-021-00554-0
pii: 10.1007/s11046-021-00554-0
pmc: PMC8249254
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
367-375Subventions
Organisme : National Natural Science Foundation of China
ID : 81602771
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