Simultaneous measurement of 13 circulating vitamin D3 and D2 mono and dihydroxy metabolites using liquid chromatography mass spectrometry.
liquid chromatography
mass spectrometry
metabolism
vitamin D
Journal
Clinical chemistry and laboratory medicine
ISSN: 1437-4331
Titre abrégé: Clin Chem Lab Med
Pays: Germany
ID NLM: 9806306
Informations de publication
Date de publication:
27 09 2021
27 09 2021
Historique:
received:
14
04
2021
accepted:
07
05
2021
pubmed:
21
5
2021
medline:
15
3
2022
entrez:
20
5
2021
Statut:
epublish
Résumé
Clinical evaluation of vitamin D status is conventionally performed by measuring serum levels of a single vitamin D metabolite, 25-hydroxyvitamin D predominantly by immunoassay methodology. However, this neglects the complex metabolic pathways involved in vitamin D bioactivity, including two canonical forms D3 and D2, bioactive 1,25-dihydroxy metabolites and inactive 24-hydroxy and other metabolites. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) can measure multiple analytes in a sample during a single run with high sensitivity and reference level specificity. We therefore aimed to develop and validate a LC-MS/MS method to measure simultaneously 13 circulating vitamin D metabolites and apply it to 103 human serum samples. The LC-MS/MS method using a Cookson-type derivatization reagent phenyl-1,2,4-triazoline-3,5-dione (PTAD) quantifies 13 vitamin D metabolites, including mono and dihydroxy-metabolites, as well as CYP11A1-derived D3 and D2 metabolites in a single run. The lower limit of quantitation was 12.5 pg/mL for 1,25(OH) This profiling method can provide new insight into circulating vitamin D metabolite pathways forming the basis for improved understanding of the role of vitamin D in health and disease.
Identifiants
pubmed: 34013677
pii: cclm-2021-0441
doi: 10.1515/cclm-2021-0441
pmc: PMC8419114
mid: NIHMS1712261
doi:
Substances chimiques
Vitamins
0
Vitamin D
1406-16-2
Cholecalciferol
1C6V77QF41
Calcifediol
P6YZ13C99Q
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
1642-1652Subventions
Organisme : BLRD VA
ID : I01 BX004293
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR071189
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR073004
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI149267
Pays : United States
Informations de copyright
© 2021 Walter de Gruyter GmbH, Berlin/Boston.
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