Blood Leukocyte Signaling Pathways as Predictors of Severity of Acute Pancreatitis.
Adult
Aged
Aged, 80 and over
Biomarkers
/ blood
Case-Control Studies
Female
Flow Cytometry
Humans
Leukocytes
/ metabolism
Male
Middle Aged
NF-kappa B
/ blood
Pancreatitis
/ blood
Phosphorylation
Predictive Value of Tests
Prospective Studies
STAT Transcription Factors
/ blood
STAT1 Transcription Factor
/ blood
STAT3 Transcription Factor
/ blood
STAT6 Transcription Factor
/ blood
Severity of Illness Index
Signal Transduction
Young Adult
Journal
Pancreas
ISSN: 1536-4828
Titre abrégé: Pancreas
Pays: United States
ID NLM: 8608542
Informations de publication
Date de publication:
Historique:
pubmed:
22
5
2021
medline:
19
1
2022
entrez:
21
5
2021
Statut:
ppublish
Résumé
Clinical practice lacks biomarkers to predict the severity of acute pancreatitis (AP). We studied if intracellular signaling of circulating leukocytes could predict persistent organ dysfunction (OD) and secondary infections in AP. A venous blood sample was taken from 174 patients with AP 72 hours or less from onset of symptoms and 31 healthy controls. Phosphorylation levels (p) of appropriately stimulated signal transducer and activator of transcription 1 (STAT1), STAT6, nuclear factor-κB (NF-κB), Akt, and nonstimulated STAT3 in monocytes, neutrophils, and lymphocytes was measured using phosphospecific flow cytometry. The patients showed higher pSTAT3 and lower pSTAT1, pSTAT6, pNF-κB, and pAkt than healthy controls. pSTAT3 in all leukocyte subtypes studied increased, and pSTAT1 in monocytes and T cells decreased in an AP severity-wise manner. In patients without OD at sampling, high pSTAT3 in monocytes and T lymphocytes were associated with development of persistent OD. In patients with OD, low interleukin-4-stimulated pSTAT6 in monocytes and neutrophils and Escherichia coli-stimulated pNF-κB in neutrophils predicted OD persistence. High pSTAT3 in monocytes, CD8+ T cells, and neutrophils; low pSTAT1 in monocytes and T cells; and low pNF-κB in lymphocytes predicted secondary infections. Leukocyte STAT3, STAT1, STAT6, and NF-κΒ phosphorylations are potential predictors of AP severity.
Identifiants
pubmed: 34016897
doi: 10.1097/MPA.0000000000001832
pii: 00006676-900000000-98255
pmc: PMC8195735
doi:
Substances chimiques
Biomarkers
0
NF-kappa B
0
STAT Transcription Factors
0
STAT1 Transcription Factor
0
STAT1 protein, human
0
STAT3 Transcription Factor
0
STAT3 protein, human
0
STAT6 Transcription Factor
0
STAT6 protein, human
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
710-718Informations de copyright
Copyright © 2021 The Author(s). Published by Wolters Kluwer Health, Inc.
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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