Nicotinamide N-methyltransferase gene silencing enhances chemosensitivity of melanoma cell lines.
Nicotinamide N-methyltransferase
cell migration
cell proliferation
chemosensitivity
cutaneous melanoma
Journal
Pigment cell & melanoma research
ISSN: 1755-148X
Titre abrégé: Pigment Cell Melanoma Res
Pays: England
ID NLM: 101318927
Informations de publication
Date de publication:
11 2021
11 2021
Historique:
revised:
11
05
2021
received:
11
03
2021
accepted:
17
05
2021
pubmed:
22
5
2021
medline:
29
3
2022
entrez:
21
5
2021
Statut:
ppublish
Résumé
Melanoma accounts for less than 5% of all cutaneous neoplasms but is responsible for the greater part of skin cancer-related deaths. Therefore, the identification of molecules that could serve as the therapeutic target is urgent. This study focused on the enzyme nicotinamide N-methyltransferase (NNMT). The effect of NNMT knockdown on cell proliferation and migration of A375 melanoma cells was evaluated by MTT and wound healing assays, respectively. Viability of A375 cells downregulating NNMT was also explored under treatment with dacarbazine, a chemotherapeutic drug approved for advanced melanoma treatment. The impact of enzyme knockdown on cell proliferation and chemosensitivity was also investigated in WM-115 melanoma cells. Results obtained demonstrated that NNMT silencing led to a significant reduction of cell proliferation and migration of A375 cells. Moreover, enzyme downregulation was associated with an increase of melanoma cells sensitivity to treatment with dacarbazine. Analogous effects induced by enzyme knockdown on cell proliferation and chemosensitivity were also found in the WM-115 cell line. Our data seem to demonstrate that NNMT could represent a promising molecular target for the effective treatment of this form of skin cancer.
Substances chimiques
Neoplasm Proteins
0
Dacarbazine
7GR28W0FJI
NNMT protein, human
EC 2.1.1.1
Nicotinamide N-Methyltransferase
EC 2.1.1.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1039-1048Informations de copyright
© 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
Références
Bach, D. H., Kim, D., Bae, S. Y., Kim, W. K., Hong, J. Y., Lee, H. J., Rajasekaran, N., Kwon, S., Fan, Y., Luu, T. T., Shin, Y. K., Lee, J., & Lee, S. K. (2018). Targeting nicotinamide N-methyltransferase and miR-449a in EGFR-TKI-resistant non-small-cell lung cancer cells. Molecular Therapy - Nucleic Acids, 11, 455-467. https://doi.org/10.1016/j.omtn.2018.03.011
Campagna, R., Cecati, M., Pozzi, V., Fumarola, S., Pompei, V., Milanese, G., Galosi, A. B., Sartini, D., & Emanuelli, M. (2018). Involvement of transforming growth factor beta 1 in the transcriptional regulation of nicotinamide N-methyltransferase in clear cell renal cell carcinoma. Cellular and Molecular Biology, 64, 51-55. https://doi.org/10.14715/cmb/2018.64.7.9
Chapman, P. B., Hauschild, A., Robert, C., Haanen, J. B., Ascierto, P., Larkin, J., Dummer, R., Garbe, C., Testori, A., Maio, M., Hogg, D., Lorigan, P., Lebbe, C., Jouary, T., Schadendorf, D., Ribas, A., O'Day, S. J., Sosman, J. A., Kirkwood, J. M., … McArthur, G. A. (2011). Improved survival with vemurafenib in melanoma with BRAF V600E mutation. New England Journal of Medicine, 364, 2507-2516. https://doi.org/10.1056/NEJMoa1103782
Coit, D. G., Thompson, J. A., Algazi, A., Andtbacka, R., Bichakjian, C. K., Carson, W. E., Daniels, G. A., DiMaio, D., Ernstoff, M., Fields, R. C., Fleming, M. D., Gonzalez, R., Guild, V., Halpern, A. C., Hodi, F. S., Joseph, R. W., Lange, J. R., Martini, M. C., Materin, M. A., … Engh, A. (2016). Melanoma, version 2.2016, NCCN clinical practice guidelines in oncology. Journal of the National Comprehensive Cancer Network, 14, 450-473. https://doi.org/10.6004/jnccn.2016.0051
Crosby, T., Fish, R., Coles, B., & Mason, M. D. (2000). Systemic treatments for metastatic cutaneous melanoma. Cochrane Database Systematic Review, 2, CD001215.
Cui, Y., Yang, D., Wang, W., Zhang, L., Liu, H., Ma, S., Guo, W., Yao, M., Zhang, K., Li, W., Zhang, Y., & Guan, F. (2020). Nicotina-mide N-methyltransferase decreases 5-fluorouracil sensitivity in human esophageal squamous cell carcinoma through metabolic reprogramming and promoting the Warburg effect. Molecular Carcinogenesis, 59, 940-954. https://doi.org/10.1002/mc.23209
Cui, Y., Zhang, L., Wang, W., Ma, S., Liu, H., Zang, X., Zhang, Y., & Guan, F. (2019). Downregulation of nicotinamide N-methyltransferase inhibits migration and epithelial-mesenchymal transition of esophageal squamous cell carcinoma via Wnt/β-catenin pathway. Molecular and Cellular Biochemistry, 460, 93-103. https://doi.org/10.1007/s11010-019-03573-0
Eggermont, A. M., & Kirkwood, J. M. (2004). Re-evaluating the role of dacarbazine in metastatic melanoma: What have we learned in 30 years? European Journal of Cancer, 40, 1825-1836. https://doi.org/10.1016/j.ejca.2004.04.030
Emanuelli, M., Santarelli, A., Sartini, D., Ciavarella, D., Rossi, V., Rubini, C., & Lo Muzio, L. (2010). Nicotinamide N-Methyltransferase upregulation correlates with tumour differentiation in oral squamous cell carcinoma. Histology and Histopathology, 25, 15-20.
Erdei, E., & Torres, S. M. (2010). A new understanding in the epidemiology of melanoma. Expert Review of Anticancer Therapy, 10, 1811-1823. https://doi.org/10.1586/era.10.170
Ganzetti, G., Sartini, D., Campanati, A., Rubini, C., Molinelli, E., Brisigotti, V., Cecati, M., Pozzi, V., Campagna, R., Offidani, A., & Emanuelli, M. (2018). Nicotinamide N-methyltransferase: Potential involvement in cutaneous malignant melanoma. Melanoma Research, 28, 82-88. https://doi.org/10.1097/CMR.0000000000000430
Leonardi, G., Falzone, L., Salemi, R., Zanghì, A., Spandidos, D. A., Mccubrey, J. A., Candido, S., & Libra, M. (2018). Cutaneous melanoma: From pathogenesis to therapy. International Journal of Oncology, 52, 1071-1080.
Liang, L., Zeng, M., Pan, H., Liu, H., & He, Y. (2018). Nicotinamide N-methyltransferase promotes epithelial-mesenchymal transition in gastric cancer cells by activating transforming growth factor-β1 expression. Oncology Letters, 15, 4592-4598. https://doi.org/10.3892/ol.2018.7885
Lui, P., Cashin, R., Machado, M., Hemels, M., Corey-Lisle, P. K., & Einarson, T. R. (2007). Treatments for metastatic melanoma: Synthesis of evidence from randomized trials. Cancer Treatment Reviews, 33, 665-680. https://doi.org/10.1016/j.ctrv.2007.06.004
Malas, S., Harrasser, M., Lacy, K. E., & Karagiannis, S. N. (2014). Antibody therapies for melanoma: New and emerging opportunities to activate immunity. Oncology Reports, 32, 875-886.
Mascitti, M., Santarelli, A., Sartini, D., Rubini, C., Colella, G., Salvolini, E., Ganzetti, G., Offidani, A., & Emanuelli, M. (2019). Analysis of nicotinamide N-methyltransferase in oral malignant melanoma and potential prognostic significance. Melanoma Research, 29, 151-156. https://doi.org/10.1097/CMR.0000000000000548
Mateuszuk, Ł., Campagna, R., Kutryb-Zając, B., Kuś, K., Słominska, E. M., Smolenski, R. T., & Chlopicki, S. (2020). Reversal of endothelial dysfunction by nicotinamide mononucleotide via extracellular conversion to nicotinamide riboside. Biochemical Pharmacology, 178, 114019. https://doi.org/10.1016/j.bcp.2020.114019
Ossio, R., Roldán-Marín, R., Martínez-Said, H., Adams, D. J., & Robles-Espinoza, C. D. (2017). Melanoma: A global perspective. Nature Reviews Cancer, 17, 393-394. https://doi.org/10.1038/nrc.2017.43
Peng, Y., Sartini, D., Pozzi, V., Wilk, D., Emanuelli, M., & Yee, V. C. (2011). Structural basis of substrate recognition in human nicotinamide N-methyltransferase. Biochemistry, 50, 7800-7808.
Pissios, P. (2017). Nicotinamide N-methyltransferase: More than a vitamin B3 clearance enzyme. Trends in Endocrinology and Metabolism, 28, 340-353. https://doi.org/10.1016/j.tem.2017.02.004
Pompei, V., Salvolini, E., Rubini, C., Lucarini, G., Molinelli, E., Brisigotti, V., Pozzi, V., Sartini, D., Campanati, A., Offidani, A., & Emanuelli, M. (2019). Nicotinamide N-methyltransferase in nonmelanoma skin cancers. European Journal of Clinical Investigation, 49, e13175. https://doi.org/10.1111/eci.13175
Pozzi, V., Di Ruscio, G., Sartini, D., Campagna, R., Seta, R., Fulvi, P., Vici, A., Milanese, G., Brandoni, G., Galosi, A. B., Montironi, R., Cecati, M., & Emanuelli, M. (2018). Clinical performance and utility of a NNMT-based urine test for bladder cancer. International Journal of Biological Markers, 33, 94-101. https://doi.org/10.5301/ijbm.5000311
Pozzi, V., Mazzotta, M., Lo Muzio, L., Sartini, D., Santarelli, A., Renzi, E., Rocchetti, R., Tomasetti, M., Ciavarella, D., & Emanuelli, M. (2011). Inhibiting proliferation in KB cancer cells by RNA interference-mediated knockdown of nicotinamide N-methyltransferase expression. International Journal of Immunopathology and Pharmacology, 24, 69-77. https://doi.org/10.1177/039463201102400109
Pozzi, V., Salvolini, E., Lucarini, G., Salvucci, A., Campagna, R., Rubini, C., Sartini, D., & Emanuelli, M. (2020). Cancer stem cell en-richment is associated with enhancement of nicotinamide N-methyltransferase expression. IUBMB Life, 72, 1415-1425. https://doi.org/10.1002/iub.2265
Pozzi, V., Sartini, D., Morganti, S., Giuliante, R., Di Ruscio, G., Santarelli, A., Rocchetti, R., Rubini, C., Tomasetti, M., Giannatempo, G., Orlando, F., Provinciali, M., Lo Muzio, L., & Emanuelli, M. (2013). RNA-mediated gene silencing of nicotinamide N-methyltransferase is associated with decreased tumorigenicity in human oral carcinoma cells. PLoS One, 8, e71272. https://doi.org/10.1371/journal.pone.0071272
Pozzi, V., Sartini, D., Rocchetti, R., Santarelli, A., Rubini, C., Morganti, S., Giuliante, R., Calabrese, S., Di Ruscio, G., Orlando, F., Provinciali, M., Saccucci, F., Lo Muzio, L., & Emanuelli, M. (2015). Identification and characterization of cancer stem cells from head and neck squamous cell carcinoma cell lines. Cellular Physiology and Biochemistry, 36, 784-798. https://doi.org/10.1159/000430138
Ross, M. I., & Gershenwald, J. E. (2011). Evidence-based treatment of early-stage melanoma. Journal of Surgical Oncology, 104, 341-353.
Sartini, D., Morganti, S., Guidi, E., Rubini, C., Zizzi, A., Giuliante, R., Pozzi, V., & Emanuelli, M. (2013). Nicotinamide N-methyltransferase in non-small cell lung cancer: Promising results for targeted anti-cancer therapy. Cell Biochemistry and Biophysics, 67, 865-873. https://doi.org/10.1007/s12013-013-9574-z
Sartini, D., Muzzonigro, G., Milanese, G., Pierella, F., Rossi, V., & Emanuelli, M. (2006). Identification of nicotinamide N-methyltransferase as a novel tumor marker for renal clear cell carcinoma. Journal of Urology, 176, 2248-2254. https://doi.org/10.1016/j.juro.2006.07.046
Sartini, D., Muzzonigro, G., Milanese, G., Pozzi, V., Vici, A., Morganti, S., Rossi, V., Mazzucchelli, R., Montironi, R., & Emanuelli, M. (2013). Upregulation of tissue and urinary nicotinamide N-methyltransferase in bladder cancer: Potential for the development of a urine-based diagnostic test. Cell Biochemistry and Biophysics, 65, 473-483. https://doi.org/10.1007/s12013-012-9451-1
Sartini, D., Pozzi, V., Renzi, E., Morganti, S., Rocchetti, R., Rubini, C., Santarelli, A., Lo Muzio, L., & Emanuelli, M. (2012). Analysis of tissue and salivary nicotinamide N-methyltransferase in oral squamous cell carcinoma: Basis for the development of a noninvasive diagnostic test for early-stage disease. Biological Chemistry, 393, 505-511. https://doi.org/10.1515/hsz-2012-0112
Sartini, D., Santarelli, A., Rossi, V., Goteri, G., Rubini, C., Ciavarella, D., Lo Muzio, L., & Emanuelli, M. (2007). Nicotinamide N-methyltransferase upregulation inversely correlates with lymph node metastasis in oral squamous cell carcinoma. Molecular Medicine, 13, 415-421. https://doi.org/10.2119/2007-00035.Sartini
Sartini, D., Seta, R., Pozzi, V., Morganti, S., Rubini, C., Zizzi, A., Tomasetti, M., Santarelli, L., & Emanuelli, M. (2015). Role of nicotinamide N-methyltransferase in non-small cell lung cancer: In vitro effect of shRNA-mediated gene silencing on tumourigenicity. Biological Chemistry, 396, 225-234. https://doi.org/10.1515/hsz-2014-0231
Seta, R., Mascitti, M., Campagna, R., Sartini, D., Fumarola, S., Santarelli, A., Giuliani, M., Cecati, M., Muzio, L. L., & Emanuelli, M. (2019). Overexpression of nicotinamide N-methyltransferase in HSC-2 OSCC cell line: Effect on apoptosis and cell proliferation. Clinical Oral Investigations, 23, 829-838. https://doi.org/10.1007/s00784-018-2497-8
Siegel, R. L., Miller, K. D., & Jemal, A. (2015). Cancer statistics, 2015. CA: A Cancer Journal for Clinicians, 65, 5-29. https://doi.org/10.3322/caac.21254
Tang, S. W., Yang, T. C., Lin, W. C., Chang, W. H., Wang, C. C., Lai, M. K., & Lin, J. Y. (2011). Nicotinamide N-methyltransferase induces cellular invasion through activating matrix metalloproteinase-2 expression in clear cell renal cell carcinoma cells. Carcinogenesis, 32, 138-145. https://doi.org/10.1093/carcin/bgq225
Ulanovskaya, O. A., Zuhl, A. M., & Cravatt, B. F. (2013). NNMT promotes epigenetic remodeling in cancer by creating a metabolic methylation sink. Nature Chemical Biology, 9, 300-306. https://doi.org/10.1038/nchembio.1204
van Haren, M. J., Taig, R., Kuppens, J., Sastre Toraño, J., Moret, E. E., Parsons, R. B., Sartini, D., Emanuelli, M., & Martin, N. I. (2017). Inhibitors of nicotinamide N-methyltransferase designed to mimic the methylation reaction transition state. Organic & Biomolecular Chemistry, 15, 6656-6667. https://doi.org/10.1039/C7OB01357D
Wang, Y., Zeng, J., Wu, W., Xie, S., Yu, H., Li, G., Zhu, T., Li, F., Lu, J., Wang, G. Y., Xie, X., & Zhang, J. (2019). Nicotinamide N-methyltransferase enhances chemoresistance in breast cancer through SIRT1 protein stabilization. Breast Cancer Research, 21, 64. https://doi.org/10.1186/s13058-019-1150-z
Wiesner, T., & Zbyszewski, A. (2018). Progress in the diagnosis and therapy of melanoma. British Journal of Dermatology, 178, 12-14. https://doi.org/10.1111/bjd.16110
Wilson, M. A., & Schuchter, L. M. (2016). Chemotherapy for melanoma. Cancer Treatment and Research, 167, 209-229.
Wu, Y., Siadaty, M. S., Berens, M. E., Hampton, G. M., & Theodorescu, D. (2008). Overlapping gene expression profiles of cell migration and tumor invasion in human bladder cancer identify metallothionein 1E and nicotinamide N-methyltransferase as novel regulators of cell migration. Oncogene, 27, 6679-6689. https://doi.org/10.1038/onc.2008.264
Xie, X., Liu, H., Wang, Y., Zhou, Y., Yu, H., Li, G., Ruan, Z., Li, F., Wang, X., & Zhang, J. (2016). Nicotinamide N-methyltransferase enhances resistance to 5-fluorouracil in colorectal cancer cells through inhibition of the ASK1-p38 MAPK pathway. Oncotarget, 7, 45837-45848. https://doi.org/10.18632/oncotarget.9962
Xie, X., Yu, H., Wang, Y., Zhou, Y., Li, G., Ruan, Z., Li, F., Wang, X., Liu, H., & Zhang, J. (2014). Nicotinamide N-methyltransferase enhances the capacity of tumorigenesis associated with the promotion of cell cycle progression in human colorectal cancer cells. Archives of Biochemistry and Biophysics, 564, 52-66. https://doi.org/10.1016/j.abb.2014.08.017
You, Z., Liu, Y., & Liu, X. (2018). Nicotinamide N-methyltransferase enhances the progression of prostate cancer by stabilizing sirtuin 1. Oncology Letters, 15, 9195-9201. https://doi.org/10.3892/ol.2018.8474
Yu, T., Wang, Y. T., Chen, P., Li, Y. H., Chen, Y. X., Zeng, H., Yu, A. M., Huang, M., & Bi, H. C. (2015). Effects of nicotinamide N-methyltransferase on PANC-1 cells proliferation, metastatic potential and survival under metabolic stress. Cellular Physiology and Biochemistry, 35, 710-721. https://doi.org/10.1159/000369731
Zhang, J., Wang, Y., Li, G., Yu, H., & Xie, X. (2014). Down-regulation of nicotinamide N-methyltransferase induces apoptosis in hu-man breast cancer cells via the mitochondria-mediated pathway. PLoS One, 9, e89202.