Modulating HIV-1 envelope glycoprotein conformation to decrease the HIV-1 reservoir.


Journal

Cell host & microbe
ISSN: 1934-6069
Titre abrégé: Cell Host Microbe
Pays: United States
ID NLM: 101302316

Informations de publication

Date de publication:
09 Jun 2021
Historique:
received: 18 08 2020
revised: 01 02 2021
accepted: 20 04 2021
pubmed: 22 5 2021
medline: 26 10 2021
entrez: 21 5 2021
Statut: ppublish

Résumé

Small CD4-mimetic compounds (CD4mc) sensitize HIV-1-infected cells to antibody-dependent cellular cytotoxicity (ADCC) by facilitating antibody recognition of epitopes that are otherwise occluded on the unliganded viral envelope (Env). Combining CD4mc with two families of CD4-induced (CD4i) antibodies, which are frequently found in plasma of HIV-1-infected individuals, stabilizes Env in a conformation that is vulnerable to ADCC. We employed new-generation SRG-15 humanized mice, supporting natural killer (NK) cell and Fc-effector functions to demonstrate that brief treatment with CD4mc and CD4i-Abs significantly decreases HIV-1 replication, the virus reservoir and viral rebound after ART interruption. These effects required Fc-effector functions and NK cells, highlighting the importance of ADCC. Viral rebound was also suppressed in HIV-1+-donor cell-derived humanized mice supplemented with autologous HIV-1+-donor-derived plasma and CD4mc. These results indicate that CD4mc could have therapeutic utility in infected individuals for decreasing the size of the HIV-1 reservoir and/or achieving a functional cure.

Identifiants

pubmed: 34019804
pii: S1931-3128(21)00190-6
doi: 10.1016/j.chom.2021.04.014
pmc: PMC8214472
mid: NIHMS1708265
pii:
doi:

Substances chimiques

Antibodies, Neutralizing 0
Antiviral Agents 0
CD4 Antigens 0
Epitopes 0
Glycoproteins 0
Immunoglobulin Fc Fragments 0
env Gene Products, Human Immunodeficiency Virus 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

904-916.e6

Subventions

Organisme : NIAID NIH HHS
ID : UM1 AI126620
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI148379
Pays : United States
Organisme : NIAID NIH HHS
ID : P01 AI150471
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI129769
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI150322
Pays : United States
Organisme : NIGMS NIH HHS
ID : P01 GM056550
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI162646
Pays : United States
Organisme : NIAID NIH HHS
ID : P01 AI131251
Pays : United States
Organisme : NIAID NIH HHS
ID : U01 AI150741
Pays : United States
Organisme : NIAID NIH HHS
ID : P50 AI150464
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI116274
Pays : United States
Organisme : NIAID NIH HHS
ID : R33 AI122384
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI145164
Pays : United States
Organisme : NIAID NIH HHS
ID : R56 AI141572
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI124982
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI050529
Pays : United States

Informations de copyright

Copyright © 2021 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

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Auteurs

Jyothi K Rajashekar (JK)

Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, USA.

Jonathan Richard (J)

Centre de Recherche du CHUM, Montreal, QC, Canada; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, Canada.

Jagadish Beloor (J)

Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, USA.

Jérémie Prévost (J)

Centre de Recherche du CHUM, Montreal, QC, Canada; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, Canada.

Sai Priya Anand (SP)

Centre de Recherche du CHUM, Montreal, QC, Canada; Department of Microbiology and Immunology, McGill University Montreal, Montreal, QC, Canada.

Guillaume Beaudoin-Bussières (G)

Centre de Recherche du CHUM, Montreal, QC, Canada; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, Canada.

Liang Shan (L)

Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA.

Dietmar Herndler-Brandstetter (D)

Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA.

Gabrielle Gendron-Lepage (G)

Centre de Recherche du CHUM, Montreal, QC, Canada.

Halima Medjahed (H)

Centre de Recherche du CHUM, Montreal, QC, Canada.

Catherine Bourassa (C)

Centre de Recherche du CHUM, Montreal, QC, Canada.

Fleur Gaudette (F)

Centre de Recherche du CHUM, Montreal, QC, Canada.

Irfan Ullah (I)

Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, USA.

Kelly Symmes (K)

Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, USA.

Andrew Peric (A)

Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, USA.

Emily Lindemuth (E)

Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Frederic Bibollet-Ruche (F)

Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Jun Park (J)

Department of Chemistry, School of Arts and Sciences, University of Pennsylvania, Philadelphia, PA, USA.

Hung-Ching Chen (HC)

Department of Chemistry, School of Arts and Sciences, University of Pennsylvania, Philadelphia, PA, USA.

Daniel E Kaufmann (DE)

Centre de Recherche du CHUM, Montreal, QC, Canada; Department of Medicine, Université de Montréal, Montreal, QC, Canada.

Beatrice H Hahn (BH)

Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Joseph Sodroski (J)

Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, and Department of Microbiology and Immunobiology, Division of AIDS, Harvard Medical School, Boston, MA, USA; Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA, USA.

Marzena Pazgier (M)

Infectious Diseases Division, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.

Richard A Flavell (RA)

Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA. Electronic address: richard.flavell@yale.edu.

Amos B Smith (AB)

Department of Chemistry, School of Arts and Sciences, University of Pennsylvania, Philadelphia, PA, USA. Electronic address: smithab@sas.upenn.edu.

Andrés Finzi (A)

Centre de Recherche du CHUM, Montreal, QC, Canada; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, Canada; Department of Microbiology and Immunology, McGill University Montreal, Montreal, QC, Canada. Electronic address: andres.finzi@umontreal.ca.

Priti Kumar (P)

Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, USA. Electronic address: priti.kumar@yale.edu.

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Classifications MeSH