Retinal ganglion cells projecting to superior colliculus and pulvinar in marmoset.

Lateral geniculate nucleus Pretectum Primate retina Pulvinar Retinal ganglion cell projections Superior colliculus

Journal

Brain structure & function
ISSN: 1863-2661
Titre abrégé: Brain Struct Funct
Pays: Germany
ID NLM: 101282001

Informations de publication

Date de publication:
Dec 2021
Historique:
received: 17 02 2021
accepted: 08 05 2021
pubmed: 23 5 2021
medline: 21 12 2021
entrez: 22 5 2021
Statut: ppublish

Résumé

We determined the retinal ganglion cell types projecting to the medial subdivision of inferior pulvinar (PIm) and the superior colliculus (SC) in the common marmoset monkey, Callithrix jacchus. Adult marmosets received a bidirectional tracer cocktail into the PIm (conjugated to Alexa fluor 488), and the SC (conjugated to Alexa fluor 594) using an MRI-guided approach. One SC injection included the pretectum. The large majority of retrogradely labelled cells were obtained from SC injections, with only a small proportion obtained after PIm injections. Retrogradely labelled cells were injected intracellularly in vitro using lipophilic dyes (DiI, DiO). The SC and PIm both received input from a variety of ganglion cell types. Input to the PIm was dominated by broad thorny (41%), narrow thorny (24%) and large bistratified (25%) ganglion cells. Input to the SC was dominated by parasol (37%), broad thorny (24%) and narrow thorny (17%) cells. Midget ganglion cells (which make up the large majority of primate retinal ganglion cells) and small bistratified (blue-ON/yellow OFF) cells were never observed to project to SC or PIm. Small numbers of other wide-field ganglion cell types were also encountered. Giant sparse (presumed melanopsin-expressing) cells were only seen following the tracer injection which included the pretectum. We note that despite the location of pulvinar complex in dorsal thalamus, and its increased size and functional importance in primate evolution, the retinal projections to pulvinar have more in common with SC projections than they do with projections to the dorsal lateral geniculate nucleus.

Identifiants

pubmed: 34021395
doi: 10.1007/s00429-021-02295-8
pii: 10.1007/s00429-021-02295-8
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

2745-2762

Subventions

Organisme : National Health and Medical Research Council
ID : APP1123418
Organisme : National Health and Medical Research Council
ID : APP1042893
Organisme : National Health and Medical Research Council
ID : APP1077677
Organisme : Centre of Excellence for Integrative Brain Function, Australian Research Council
ID : CE140100007

Informations de copyright

© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

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Auteurs

Ulrike Grünert (U)

Save Sight Institute, Discipline of Clinical Ophthalmology, Sydney Medical School, The University of Sydney, 8 Macquarie Street, Sydney, NSW, 2000, Australia. ulrike.grunert@sydney.edu.au.
Australian Research Council Centre of Excellence for Integrative Brain Function, Sydney Node, The University of Sydney, Sydney, NSW, 2000, Australia. ulrike.grunert@sydney.edu.au.

Sammy C S Lee (SCS)

Save Sight Institute, Discipline of Clinical Ophthalmology, Sydney Medical School, The University of Sydney, 8 Macquarie Street, Sydney, NSW, 2000, Australia.
Australian Research Council Centre of Excellence for Integrative Brain Function, Sydney Node, The University of Sydney, Sydney, NSW, 2000, Australia.

William C Kwan (WC)

Australian Regenerative Medicine Institute, Monash University, Clayton, VIC, Australia.

Inaki-Carril Mundinano (IC)

Australian Regenerative Medicine Institute, Monash University, Clayton, VIC, Australia.

James A Bourne (JA)

Australian Regenerative Medicine Institute, Monash University, Clayton, VIC, Australia.

Paul R Martin (PR)

Save Sight Institute, Discipline of Clinical Ophthalmology, Sydney Medical School, The University of Sydney, 8 Macquarie Street, Sydney, NSW, 2000, Australia.
Australian Research Council Centre of Excellence for Integrative Brain Function, Sydney Node, The University of Sydney, Sydney, NSW, 2000, Australia.

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