Overexpression of miRNA-9 enhances galectin-3 levels in oral cavity cancers.
Apoptosis
/ genetics
Blood Proteins
/ genetics
Cell Line, Tumor
Cell Movement
/ genetics
Cell Proliferation
/ genetics
Female
Galectin 3
/ genetics
Galectins
/ genetics
Gene Expression
/ genetics
Gene Expression Profiling
/ methods
Gene Expression Regulation, Neoplastic
/ genetics
Head and Neck Neoplasms
/ genetics
Humans
Male
MicroRNAs
/ genetics
Mouth
Mouth Neoplasms
/ genetics
Saliva
/ chemistry
Transcriptome
/ genetics
AKT
Galectin-3
MMP
Oral cavity cancer
miR-9-5p
γ-catenin
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
May 2021
May 2021
Historique:
received:
01
02
2021
accepted:
03
05
2021
pubmed:
23
5
2021
medline:
15
12
2021
entrez:
22
5
2021
Statut:
ppublish
Résumé
Oral cavity cancer (OCC) is the predominant subtype of head and neck cancer (HNC) and has up to 50% mortality. Genome-wide microRNA (miR) sequencing data indicates overexpression of miR-9-5p in HNC tumours, however, the biological role of miR-9-5p in OCC is complex; it can either act as a tumour suppressor or an oncomir, regulating many target genes at the post-transcriptional level. We have investigated the overexpression of miR-9-5p in three OCC cell lines. We have evaluated its expression levels and Galectin-3 as potential biomarkers in saliva samples collected from controls and OCC patients. We found that over expression of miR-9-5p in OCC cell lines resulted in a significant reduction in cell proliferation and migration, and an increase in apoptosis, which was paralleled by an increase in Galectin-3 secretion and export of Galectin-3 protein. Our data are consistent with miR-9-5p being a modulator of Galectin-3 via the AKT/γ-catenin pathway. In addition, the positive correlation between the levels of miR-9-5p expression and secreted Galectin-3 in saliva reflects a similar relationship in vivo, and supports the utility of their integrative evaluation in OCC. Our findings indicate that both miR-9-5p and Galectin-3 are critical biomolecules in the progression of OCC.
Identifiants
pubmed: 34021445
doi: 10.1007/s11033-021-06398-7
pii: 10.1007/s11033-021-06398-7
doi:
Substances chimiques
Blood Proteins
0
Galectin 3
0
Galectins
0
LGALS3 protein, human
0
MIRN92 microRNA, human
0
MicroRNAs
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3979-3989Subventions
Organisme : Cancer Australia
ID : APP1145657
Organisme : National Health and Medical Research Council
ID : APP2002576
Organisme : National Health Institute
ID : 1R21EB030349-01
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