Fine Sampling of Sequence Space for Membrane Protein Structural Biology.

Amino Acid Sequence Bacteria / genetics Bacterial Proteins / chemistry Genomics Membrane Proteins / chemistry Models, Molecular Protein Conformation
high throughput biology integral membrane proteins protein expression protein purification structural biology structural genomics

Journal

Journal of molecular biology
ISSN: 1089-8638
Titre abrégé: J Mol Biol
Pays: Netherlands
ID NLM: 2985088R

Informations de publication

Date de publication:
23 07 2021
Historique:
received: 26 03 2021
revised: 12 05 2021
accepted: 12 05 2021
pubmed: 23 5 2021
medline: 21 9 2021
entrez: 22 5 2021
Statut: ppublish

Résumé

We describe an enhancement of traditional genomics-based approaches to improve the success of structure determination of membrane proteins. Following a broad screen of sequence space to identify initial expression-positive targets, we employ a second step to select orthologs with closely related sequences to these hits. We demonstrate that a greater percentage of these latter targets express well and are stable in detergent, increasing the likelihood of identifying candidates that will ultimately yield structural information.

Identifiants

DOI: 10.1016/j.jmb.2021.167055 PMID: 34022208 PMC: PMC8286341
pubmed: 34022208
pii: S0022-2836(21)00273-4
doi: 10.1016/j.jmb.2021.167055
pmc: PMC8286341
mid: NIHMS1706670
pii:
doi:

Substances chimiques

Bacterial Proteins 0
Membrane Proteins 0

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

167055

Subventions

Organisme : NIGMS NIH HHS
ID : R35 GM132120
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM098617
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM107462
Pays : United States
Organisme : NIGMS NIH HHS
ID : P41 GM116799
Pays : United States
Organisme : NIGMS NIH HHS
ID : U54 GM095315
Pays : United States

Informations de copyright

Copyright © 2021 Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Competing Interests The authors declare no competing interests.

Références

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Auteurs

Michael Loukeris (M)

Center on Membrane Protein Production and Analysis (COMPPÅ), New York Structural Biology Center (NYSBC), New York, NY 10027, USA.

Zahra Assur Sanghai (ZA)

Rockefeller University, New York, NY 10065, USA.

Jeremie Vendome (J)

Schrödinger, Inc., New York, NY 10036, USA.

Wayne A Hendrickson (WA)

Department of Biochemistry and Molecular Biophysics, Columbia University Irving Medical Center, New York, NY 10032, USA; Department of Physiology and Cellular Biophysics, Columbia University Irving Medical Center, New York, NY 10032, USA.

Brian Kloss (B)

Center on Membrane Protein Production and Analysis (COMPPÅ), New York Structural Biology Center (NYSBC), New York, NY 10027, USA. Electronic address: bkloss@nysbc.org.

Filippo Mancia (F)

Department of Physiology and Cellular Biophysics, Columbia University Irving Medical Center, New York, NY 10032, USA. Electronic address: fm123@cumc.columbia.edu.

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Classifications MeSH

questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence d'acides aminés › Fine Sampling of Sequence Space for Membrane...
questionsmedicales.fr › Bactéries › Fine Sampling of Sequence Space for Membrane...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Fine Sampling of Sequence Space for Membrane...
questionsmedicales.fr › Disciplines des sciences naturelles › Disciplines des sciences biologiques › Biologie › Génétique › Génomique › Fine Sampling of Sequence Space for Membrane...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Fine Sampling of Sequence Space for Membrane...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles moléculaires › Fine Sampling of Sequence Space for Membrane...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Fine Sampling of Sequence Space for Membrane...