Sampling forest soils to describe fungal diversity and composition. Which is the optimal sampling size in mediterranean pure and mixed pine oak forests?

Biodiversity DNA, Fungal / genetics Environmental Monitoring / methods Forests Fungi / classification Pinus / microbiology Quercus / microbiology Soil Microbiology
Beta-diversity DNA metabarcoding Fungal communities Mediterranean forest Mixed forests Sample pooling

Journal

Fungal biology
ISSN: 1878-6146
Titre abrégé: Fungal Biol
Pays: Netherlands
ID NLM: 101524465

Informations de publication

Date de publication:
06 2021
Historique:
received: 15 06 2020
revised: 14 01 2021
accepted: 18 01 2021
entrez: 24 5 2021
pubmed: 25 5 2021
medline: 4 9 2021
Statut: ppublish

Résumé

Soil sampling is a critical step affecting perceived fungal diversity, however sampling optimization for high-throughput-DNA sequencing studies have never been tested in Mediterranean forest ecosystems. We identified the minimum number of pooled samples needed to obtain a reliable description of fungal communities in terms of diversity and composition in three different Mediterranean forests (pine, oak, and mixed-pine-oak). Twenty soil samples were randomly selected in each of the three plots per type. Samples obtained in 100 m

Identifiants

DOI: 10.1016/j.funbio.2021.01.005 PMID: 34024594
pubmed: 34024594
pii: S1878-6146(21)00012-X
doi: 10.1016/j.funbio.2021.01.005
pii:
doi:

Substances chimiques

DNA, Fungal 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

469-476

Informations de copyright

Copyright © 2021 British Mycological Society. Published by Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare they have no conflict of interest.

Auteurs

Irene Adamo (I)

Joint Research Unit CTFC - AGROTECNIO, Av. Alcalde Rovira Roure 191, E25198, Lleida, Spain; Dep. Crop and Forest Sciences, University of Lleida, Av. Alcalde Rovira Roure 191, E25198, Lleida, Spain. Electronic address: irene.adamo@udl.cat.

Yasmine Piñuela (Y)

Joint Research Unit CTFC - AGROTECNIO, Av. Alcalde Rovira Roure 191, E25198, Lleida, Spain; Dep. Crop and Forest Sciences, University of Lleida, Av. Alcalde Rovira Roure 191, E25198, Lleida, Spain; Forest Science and Technology Centre of Catalonia, Ctra, Sant Llorenç de Morunys Km 2, E25280, Solsona, Spain.

José Antonio Bonet (JA)

Joint Research Unit CTFC - AGROTECNIO, Av. Alcalde Rovira Roure 191, E25198, Lleida, Spain; Dep. Crop and Forest Sciences, University of Lleida, Av. Alcalde Rovira Roure 191, E25198, Lleida, Spain.

Carles Castaño (C)

Swedish University of Agricultural Sciences, Department of Forest Mycology and Plant Pathology, SE, 75007, Uppsala, Sweden.

Juan Martínez de Aragón (J)

Joint Research Unit CTFC - AGROTECNIO, Av. Alcalde Rovira Roure 191, E25198, Lleida, Spain; Forest Science and Technology Centre of Catalonia, Ctra, Sant Llorenç de Morunys Km 2, E25280, Solsona, Spain.

Javier Parladé (J)

Sustainable Plant Protection, IRTA, Centre de Cabrils, Ctra Cabrils Km 2, E08348, Cabrils, Barcelona, Spain.

Joan Pera (J)

Sustainable Plant Protection, IRTA, Centre de Cabrils, Ctra Cabrils Km 2, E08348, Cabrils, Barcelona, Spain.

Josu G Alday (JG)

Joint Research Unit CTFC - AGROTECNIO, Av. Alcalde Rovira Roure 191, E25198, Lleida, Spain; Dep. Crop and Forest Sciences, University of Lleida, Av. Alcalde Rovira Roure 191, E25198, Lleida, Spain.

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Classifications MeSH

questionsmedicales.fr › Environnement et santé publique › Environnement › Écosystème › Biodiversité › Sampling forest soils to describe fungal...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › ADN fongique › Sampling forest soils to describe fungal...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Pratiques en santé publique › Surveillance de l'environnement › Sampling forest soils to describe fungal...
questionsmedicales.fr › Environnement et santé publique › Environnement › Écosystème › Forêts › Sampling forest soils to describe fungal...
questionsmedicales.fr › Eucaryotes › Champignons › Sampling forest soils to describe fungal...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Pinales › Pinaceae › Pinus › Sampling forest soils to describe fungal...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Rosanae › Fagales › Fagaceae › Quercus › Sampling forest soils to describe fungal...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Microbiologie de l'environnement › Microbiologie du sol › Sampling forest soils to describe fungal...