Gas Plasma Technology Augments Ovalbumin Immunogenicity and OT-II T Cell Activation Conferring Tumor Protection in Mice.
Animals
Cancer Vaccines
/ immunology
Cell Line, Tumor
Disease Models, Animal
Inflammation
/ immunology
Lymphocyte Activation
/ immunology
Melanoma
/ drug therapy
Mice
Mice, Inbred C57BL
Ovalbumin
/ chemistry
Oxidative Stress
Plasma Gases
/ chemistry
Reactive Oxygen Species
/ metabolism
T-Lymphocytes
/ immunology
ROS
kINPen
ovalbumin
oxPTM
vaccines
Journal
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
ISSN: 2198-3844
Titre abrégé: Adv Sci (Weinh)
Pays: Germany
ID NLM: 101664569
Informations de publication
Date de publication:
05 2021
05 2021
Historique:
received:
04
09
2020
revised:
22
01
2021
entrez:
24
5
2021
pubmed:
25
5
2021
medline:
5
1
2022
Statut:
epublish
Résumé
Reactive oxygen species (ROS/RNS) are produced during inflammation and elicit protein modifications, but the immunological consequences are largely unknown. Gas plasma technology capable of generating an unmatched variety of ROS/RNS is deployed to mimic inflammation and study the significance of ROS/RNS modifications using the model protein chicken ovalbumin (Ova vs oxOva). Dynamic light scattering and circular dichroism spectroscopy reveal structural modifications in oxOva compared to Ova. T cells from Ova-specific OT-II but not from C57BL/6 or SKH-1 wild type mice presents enhanced activation after Ova addition. OxOva exacerbates this activation when administered ex vivo or in vivo, along with an increased interferon-gamma production, a known anti-melanoma agent. OxOva vaccination of wild type mice followed by inoculation of syngeneic B16F10 Ova-expressing melanoma cells shows enhanced T cell number and activation, decreased tumor burden, and elevated numbers of antigen-presenting cells when compared to their Ova-vaccinated counterparts. Analysis of oxOva using mass spectrometry identifies three hot spots regions rich in oxidative modifications that are associated with the increased T cell activation. Using Ova as a model protein, the findings suggest an immunomodulating role of multi-ROS/RNS modifications that may spur novel research lines in inflammation research and for vaccination strategies in oncology.
Identifiants
pubmed: 34026437
doi: 10.1002/advs.202003395
pii: ADVS2461
pmc: PMC8132054
doi:
Substances chimiques
Cancer Vaccines
0
Plasma Gases
0
Reactive Oxygen Species
0
Ovalbumin
9006-59-1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2003395Informations de copyright
© 2021 The Authors. Advanced Science published by Wiley‐VCH GmbH.
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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