A dual-model "on-super off" photoelectrochemical/ratiometric electrochemical biosensor for ultrasensitive and accurate detection of microRNA-224.

Biosensing Techniques DNA Electrochemical Techniques Limit of Detection MicroRNAs Nucleic Acid Amplification Techniques
Dual-model MicroRNA-224 Photoelectrochemical biosensor Ratiometric electrochemical biosensor Signal amplification strategies

Journal

Biosensors & bioelectronics
ISSN: 1873-4235
Titre abrégé: Biosens Bioelectron
Pays: England
ID NLM: 9001289

Informations de publication

Date de publication:
15 Sep 2021
Historique:
received: 10 02 2021
revised: 22 04 2021
accepted: 11 05 2021
pubmed: 25 5 2021
medline: 12 6 2021
entrez: 24 5 2021
Statut: ppublish

Résumé

A dual-model "on-super off" photoelectrochemical (PEC)/ratiometric electrochemical (EC) biosensor based on signal enhancing and quenching combining three-dimensional (3D) DNA walker strategy was designed for the ultrasensitive and accurate detection of microRNA-224 (miRNA-224). The "signal on" PEC state was achieved by methylene blue labeled hairpin DNA (MB-DNA) for sensitizing CdS QDs. Then numerous transformational ferrocene labeled DNAs (Fc-DNAs) converted by target-induced 3D DNA walker amplification with the help of Ag nanocubes (NCs) label DNA (Ag-DNA) were introduced to open hairpin MB-DNA. Such configuration change would relocate the sensitizer MB and the quencher Fc, whereas energy transfer placed between Ag NCs and CdS QDs, thereby significantly quenching the PEC signal to obtain "super off" state. Meanwhile, these changes resulted in a decreased oxidation peak current of MB (I

Identifiants

DOI: 10.1016/j.bios.2021.113337 PMID: 34030091
pubmed: 34030091
pii: S0956-5663(21)00374-2
doi: 10.1016/j.bios.2021.113337
pii:
doi:

Substances chimiques

MicroRNAs 0
DNA 9007-49-2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

113337

Informations de copyright

Copyright © 2021 Elsevier B.V. All rights reserved.

Auteurs

Ruiying Yang (R)

College of Public Health, Zhengzhou University, Zhengzhou, 450001, China.

Guihua Jiang (G)

College of Chemistry, Zhengzhou University, Zhengzhou, 450001, China.

Huimin Liu (H)

College of Public Health, Zhengzhou University, Zhengzhou, 450001, China.

Leiliang He (L)

College of Public Health, Zhengzhou University, Zhengzhou, 450001, China.

Fei Yu (F)

College of Public Health, Zhengzhou University, Zhengzhou, 450001, China.

Li'e Liu (L)

College of Public Health, Zhengzhou University, Zhengzhou, 450001, China.

Lingbo Qu (L)

Institute of Green Catalysis, College of Chemistry, Zhengzhou University, Zhengzhou, 450001, China.

Yongjun Wu (Y)

College of Public Health, Zhengzhou University, Zhengzhou, 450001, China; College of Chemistry, Zhengzhou University, Zhengzhou, 450001, China. Electronic address: wuyongjun135@126.com.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Techniques de biocapteur › A dual-model "on-super off"...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › A dual-model "on-super off"...
questionsmedicales.fr › Techniques d'investigation › Techniques électrochimiques › A dual-model "on-super off"...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Statistiques comme sujet › Sensibilité et spécificité › Limite de détection › A dual-model "on-super off"...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › Petit ARN non traduit › microARN › A dual-model "on-super off"...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › A dual-model "on-super off"...