Characterization of histone inheritance patterns in the Drosophila female germline.
Drosophila
FISH
asymmetric cell division
epigenetics
histone
Journal
EMBO reports
ISSN: 1469-3178
Titre abrégé: EMBO Rep
Pays: England
ID NLM: 100963049
Informations de publication
Date de publication:
05 07 2021
05 07 2021
Historique:
revised:
02
04
2021
received:
17
08
2020
accepted:
16
04
2021
pubmed:
26
5
2021
medline:
12
8
2021
entrez:
25
5
2021
Statut:
ppublish
Résumé
Stem cells have the unique ability to undergo asymmetric division which produces two daughter cells that are genetically identical, but commit to different cell fates. The loss of this balanced asymmetric outcome can lead to many diseases, including cancer and tissue dystrophy. Understanding this tightly regulated process is crucial in developing methods to treat these abnormalities. Here, we report that during a Drosophila female germline stem cell asymmetric division, the two daughter cells differentially inherit histones at key genes related to either maintaining the stem cell state or promoting differentiation, but not at constitutively active or silenced genes. We combine histone labeling with DNA Oligopaints to distinguish old versus new histones and visualize their inheritance patterns at a single-gene resolution in asymmetrically dividing cells in vivo. This strategy can be applied to other biological systems involving cell fate change during development or tissue homeostasis in multicellular organisms.
Identifiants
pubmed: 34031963
doi: 10.15252/embr.202051530
pmc: PMC8406404
doi:
Substances chimiques
Drosophila Proteins
0
Histones
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e51530Subventions
Organisme : NIGMS NIH HHS
ID : R01GM112008
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM112008
Pays : United States
Organisme : NEI NIH HHS
ID : R01EY025598
Pays : United States
Organisme : NEI NIH HHS
ID : F31 EY026786
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM127075
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY025598
Pays : United States
Organisme : NIDDK NIH HHS
ID : F31DK122702
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35GM127075
Pays : United States
Organisme : NIGMS NIH HHS
ID : F31 GM122339
Pays : United States
Organisme : NIGMS NIH HHS
ID : F31GM122339
Pays : United States
Organisme : NIDDK NIH HHS
ID : F31 DK122702
Pays : United States
Organisme : Howard Hughes Medical Institute
ID : 55108512
Pays : United States
Organisme : NEI NIH HHS
ID : F31EY026786
Pays : United States
Informations de copyright
© 2021 The Authors. Published under the terms of the CC BY NC ND 4.0 license.
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