Optogenetic strategies for high-efficiency all-optical interrogation using blue-light-sensitive opsins.
CoChR
jRCaMP1a
mouse
mouse cortex
neuroscience
red-shifted functional indicators
soma targeting
two-photon optogenetics
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
25 05 2021
25 05 2021
Historique:
received:
22
09
2020
accepted:
24
05
2021
pubmed:
26
5
2021
medline:
27
10
2021
entrez:
25
5
2021
Statut:
epublish
Résumé
All-optical methods for imaging and manipulating brain networks with high spatial resolution are fundamental to study how neuronal ensembles drive behavior. Stimulation of neuronal ensembles using two-photon holographic techniques requires high-sensitivity actuators to avoid photodamage and heating. Moreover, two-photon-excitable opsins should be insensitive to light at wavelengths used for imaging. To achieve this goal, we developed a novel soma-targeted variant of the large-conductance blue-light-sensitive opsin CoChR (stCoChR). In the mouse cortex in vivo, we combined holographic two-photon stimulation of stCoChR with an amplified laser tuned at the opsin absorption peak and two-photon imaging of the red-shifted indicator jRCaMP1a. Compared to previously characterized blue-light-sensitive soma-targeted opsins in vivo, stCoChR allowed neuronal stimulation with more than 10-fold lower average power and no spectral crosstalk. The combination of stCoChR, tuned amplified laser stimulation, and red-shifted functional indicators promises to be a powerful tool for large-scale interrogation of neural networks in the intact brain.
Identifiants
pubmed: 34032211
doi: 10.7554/eLife.63359
pii: 63359
pmc: PMC8177884
doi:
pii:
Substances chimiques
Opsins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NINDS NIH HHS
ID : U19 NS107464
Pays : United States
Organisme : NINDS NIH HHS
ID : U01 NS090576
Pays : United States
Informations de copyright
© 2021, Forli et al.
Déclaration de conflit d'intérêts
AF, MP, YP, OY, TF No competing interests declared
Références
Cell. 1984 Dec;39(3 Pt 2):499-509
pubmed: 6096007
Proc Natl Acad Sci U S A. 2003 Nov 25;100(24):13940-5
pubmed: 14615590
Elife. 2017 May 16;6:
pubmed: 28509666
Nat Methods. 2005 Dec;2(12):932-40
pubmed: 16299478
Neuron. 2020 Oct 28;108(2):382-393.e5
pubmed: 32841590
Nature. 2013 Jul 18;499(7458):295-300
pubmed: 23868258
Nat Neurosci. 2019 Jul;22(7):1148-1158
pubmed: 31110324
Proc Natl Acad Sci U S A. 2020 Sep 8;117(36):22532-22543
pubmed: 32848058
Cell Rep. 2018 Jul 31;24(5):1243-1253.e5
pubmed: 30067979
Science. 2018 Jan 26;359(6374):463-465
pubmed: 29371470
Nat Rev Neurosci. 2001 Aug;2(8):539-50
pubmed: 11483997
Cell Rep. 2017 Jul 11;20(2):333-343
pubmed: 28700936
PLoS One. 2011;6(4):e18556
pubmed: 21602908
Nat Neurosci. 2013 Oct;16(10):1499-508
pubmed: 23995068
Nat Neurosci. 2017 Dec;20(12):1796-1806
pubmed: 29184208
J Neurosci. 2017 Nov 1;37(44):10679-10689
pubmed: 28972125
Nat Rev Neurosci. 2015 Aug;16(8):487-97
pubmed: 26152865
Biophys J. 2014 Oct 7;107(7):1554-63
pubmed: 25296307
Science. 2015 Aug 7;349(6248):647-50
pubmed: 26113638
Biomed Opt Express. 2018 Apr 23;9(5):2304-2311
pubmed: 29760989
Cell Rep. 2020 Feb 25;30(8):2567-2580.e6
pubmed: 32101736
Annu Rev Biophys. 2017 May 22;46:271-293
pubmed: 28301770
Elife. 2016 Mar 24;5:
pubmed: 27011354
Science. 2019 Aug 9;365(6453):
pubmed: 31320556
Nature. 2019 Jan;565(7741):645-649
pubmed: 30651638
Cereb Cortex. 2016 Apr;26(4):1778-94
pubmed: 26819275
Nat Methods. 2014 Mar;11(3):338-46
pubmed: 24509633
Nat Rev Neurosci. 2006 May;7(5):358-66
pubmed: 16760916
Front Cell Neurosci. 2016 Oct 18;10:234
pubmed: 27803649
Nat Neurosci. 2014 Dec;17(12):1816-24
pubmed: 25402854
Elife. 2016 Aug 15;5:
pubmed: 27525487
Nat Methods. 2012 Dec;9(12):1171-9
pubmed: 23169303
Neuron. 2016 Jan 20;89(2):285-99
pubmed: 26774160
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Curr Biol. 2019 May 6;29(9):1481-1490.e6
pubmed: 31031117
Nat Methods. 2015 Feb;12(2):140-6
pubmed: 25532138
Sci Rep. 2017 Jan 05;7:40041
pubmed: 28053310
J Neurophysiol. 2016 Sep 1;116(3):1012-23
pubmed: 27281749
J Neurosci. 2015 Oct 14;35(41):13917-26
pubmed: 26468193
Science. 2016 Aug 12;353(6300):691-4
pubmed: 27516599
Nat Neurosci. 2019 Jul;22(7):1061-1065
pubmed: 31209378
J Neurosci. 2019 May 1;39(18):3484-3497
pubmed: 30833505
Opt Lett. 2011 Sep 1;36(17):3503-5
pubmed: 21886258
Cell Rep. 2018 Mar 13;22(11):3087-3098
pubmed: 29539433
Nature. 2019 Mar;567(7748):334-340
pubmed: 30842660
Curr Biol. 2020 May 4;30(9):1589-1599.e10
pubmed: 32169206
Nat Commun. 2018 Oct 8;9(1):4125
pubmed: 30297821
Opt Express. 2010 Aug 30;18(18):18720-31
pubmed: 20940765
J Neurosci Methods. 2015 Feb 15;241:66-77
pubmed: 25497065
Neuron. 2017 Feb 8;93(3):491-507
pubmed: 28182905
Nature. 2011 Jul 27;477(7363):171-8
pubmed: 21796121
Nat Neurosci. 2018 Jun;21(6):881-893
pubmed: 29713079
Elife. 2018 Feb 07;7:
pubmed: 29412138
Nat Methods. 2012 Dec;9(12):1202-5
pubmed: 23142873
Nature. 2009 Jun 4;459(7247):698-702
pubmed: 19396159
Nat Neurosci. 2013 Feb;16(2):227-34
pubmed: 23313909
Cell. 2019 Jul 11;178(2):447-457.e5
pubmed: 31257030
Neural Comput. 2006 Aug;18(8):1951-86
pubmed: 16771659
Neuron. 2000 Feb;25(2):385-97
pubmed: 10719893