Enhanced migration of engrafted retinal progenitor cells into the host retina via disruption of glial barriers.
Animals
Blotting, Western
Cell Movement
/ drug effects
Cell Survival
Cells, Cultured
Chondroitin ABC Lyase
/ pharmacology
Glial Fibrillary Acidic Protein
/ metabolism
Green Fluorescent Proteins
/ metabolism
Mice
Mice, Inbred C57BL
Neuroglia
/ metabolism
Retina
/ cytology
Stem Cell Transplantation
Stem Cells
/ cytology
Vimentin
/ metabolism
Journal
Molecular vision
ISSN: 1090-0535
Titre abrégé: Mol Vis
Pays: United States
ID NLM: 9605351
Informations de publication
Date de publication:
2021
2021
Historique:
received:
07
04
2017
accepted:
08
05
2021
entrez:
26
5
2021
pubmed:
27
5
2021
medline:
15
12
2021
Statut:
epublish
Résumé
Migration and integration remain critical challenges for stem cell replacement therapy. Glial barriers play an important role in preventing cell migration and integration. The purpose of this study was to investigate the effect and mechanisms of chondroitinase ABC on the migration of murine retinal progenitor cells (mRPCs) transplanted into the subretinal space of B6 mice. mRPCs were harvested from the neural retinas of P1 enhanced green fluorescent protein (GFP) B6 mice. Two μl containing 2 × 10 Glial fibrillary acidic protein (GFAP) and vimentin could be seen around the transplanted mRPCs in the B6 mice. Formation of glial barriers prevented the migration of donor cells into the retinal layers. Chondroitinase ABC promoted the migration and survival rates of the engrafted retinal progenitor cells in the retinal layers of recipient B6 mice. Injection induced upregulation of GFAP, chondroitin, and CD44 expression. Chondroitinase ABC disrupted the glial barriers. The CD44 around the mRPCs was much lower in the chondroitinase group. However, the CD44 in the retinal layers was considerably higher in the chondroitinase group. With the employment of chondroitinase ABC, more cells migrated into the outer nuclear layer or inner nuclear layer. The chondroitin and CD44 expression decreased 3 weeks after transplantation in the chondroitinase ABC group. Chondroitinase ABC degraded glial barriers and enhanced the migration of transplanted mouse retinal progenitor cells. Chondroitinase ABC may also have induced activation of the CD44 signaling pathway to exert the effect.
Substances chimiques
Glial Fibrillary Acidic Protein
0
Vim protein, mouse
0
Vimentin
0
enhanced green fluorescent protein
0
glial fibrillary astrocytic protein, mouse
0
Green Fluorescent Proteins
147336-22-9
Chondroitin ABC Lyase
EC 4.2.2.20
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
300-308Informations de copyright
Copyright © 2021 Molecular Vision.
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