Novel application of indocyanine green fluorescence imaging for real-time detection of thrombus in a membrane oxygenator.
acute animal experiment
coronavirus disease 2019
extracorporeal membrane oxygenation
indocyanine green
indocyanine green fluorescence imaging
oxygenator
real-time thrombus detection
thrombus
Journal
Artificial organs
ISSN: 1525-1594
Titre abrégé: Artif Organs
Pays: United States
ID NLM: 7802778
Informations de publication
Date de publication:
Oct 2021
Oct 2021
Historique:
revised:
17
04
2021
received:
01
03
2021
accepted:
11
05
2021
pubmed:
27
5
2021
medline:
16
9
2021
entrez:
26
5
2021
Statut:
ppublish
Résumé
Extracorporeal membrane oxygenation (ECMO) plays an important role in the coronavirus disease 2019 (COVID-19) pandemic. Management of thrombi in ECMO is generally an important issue; especially in ECMO for COVID-19 patients who are prone to thrombus formation, the thrombus formation in oxygenators is an unresolved issue, and it is very difficult to deal with. To prevent thromboembolic complications, it is necessary to develop a method for early thrombus detection. We developed a novel method for detailed real-time observation of thrombi formed in oxygenators using indocyanine green (ICG) fluorescence imaging. The purpose of this study was to verify the efficacy of this novel method through animal experiments. The experiments were performed three times using three pigs equipped with veno-arterial ECMO comprising a centrifugal pump (CAPIOX SL) and an oxygenator (QUADROX). To create thrombogenic conditions, the pump flow rate was set at 1 L/min without anticoagulation. The diluted ICG (0.025 mg/mL) was intravenously administered at a dose of 10 mL once an hour. A single dose of ICG was 0.25mg. The oxygenator was observed with both an optical detector (PDE-neo) and the naked eye every hour after measurement initiation for a total of 8 hours. With this dose of ICG, we could observe it by fluorescence imaging for about 15 minutes. Under ICG imaging, the inside of the oxygenator was observed as a white area. A black dot suspected to be the thrombus appeared 6-8 hours after measurement initiation. The thrombus and the black dot on ICG imaging were finely matched in terms of morphology. Thus, we succeeded in real-time thrombus detection in an oxygenator using ICG imaging. The combined use of ICG imaging and conventional routine screening tests could compensate for each other's weaknesses and significantly improve the safety of ECMO.
Substances chimiques
Fluorescent Dyes
0
Indocyanine Green
IX6J1063HV
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1173-1182Subventions
Organisme : Japan Agency for Medical Research and Development
ID : 20338610
Organisme : TERUMO LIFE SCIENCE FOUNDATION
ID : 20-107
Informations de copyright
© 2021 International Center for Artificial Organs and Transplantation and Wiley Periodicals, Inc.
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