Constitutive Endocytosis of the Neuronal Glutamate Transporter Excitatory Amino Acid Transporter-3 Requires ARFGAP1.
ARF6
ARFGAP1
endocytosis
excitatory amino acid transporter-3
regulated recycling
Journal
Frontiers in physiology
ISSN: 1664-042X
Titre abrégé: Front Physiol
Pays: Switzerland
ID NLM: 101549006
Informations de publication
Date de publication:
2021
2021
Historique:
received:
22
02
2021
accepted:
13
04
2021
entrez:
27
5
2021
pubmed:
28
5
2021
medline:
28
5
2021
Statut:
epublish
Résumé
The eukaryotic endocytic pathway regulates protein levels available at the plasma membrane by recycling them into specific endosomal compartments. ARFGAP1 is a component of the coat protein I (COPI) complex but it also plays a role in promoting adapter protein-2 (AP-2) mediated endocytosis. The excitatory amino acid transporter-3 (EAAT3) mediates the reuptake of glutamate from the synaptic cleft to achieve rapid termination of synaptic transmission at glutamatergic synapses. In this study, we identified two interacting proteins of EAAT3 by mass spectrometry (MS) ARFGAP1 and ARF6. We explored the role of ARFGAP1 and ARF6 in the endocytosis of EAAT3. Our data revealed that ARFGAP1 plays a role in the recycling of EAAT3, by utilizing its GTPase activating protein (GAP) activity and ARF6 acting as the substrate. ARFGAP1 promotes cargo sorting of EAAT3
Identifiants
pubmed: 34040545
doi: 10.3389/fphys.2021.671034
pmc: PMC8141794
doi:
Types de publication
Journal Article
Langues
eng
Pagination
671034Subventions
Organisme : Austrian Science Fund FWF
ID : P 31255
Pays : Austria
Organisme : Austrian Science Fund FWF
ID : W 1232
Pays : Austria
Informations de copyright
Copyright © 2021 Saha, Yang, Hofmaier, Venkatesan, Steinkellner, Kudlacek, Sucic, Freissmuth and Sitte.
Déclaration de conflit d'intérêts
HS has received honoraria for lectures and consulting from AbbVie, Amgen, Astropharma, Astra Zeneca, Bano Healthcare, Chiesi, FOPI, Gebro, IIR, Lundbeck, MSD, Novartis, Pfizer, Roche, Rokitan, Sanofi-Aventis, Shire, and Vertex. MF has received honoraria for lectures and consulting from Amgen, Astra Zeneca, Astropharma, Baxter, Boehringer-Ingelheim, Celgene, Lundbeck, Merck-Sharp & Dohme, Novartis-Sandoz, Ratiopharm, and the Association of Austrian Sickness Funds. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Traffic. 2015 Dec;16(12):1210-38
pubmed: 26403691
Ann Neurol. 2011 Mar;69(3):509-20
pubmed: 21446024
J Pharmacol Sci. 2008 Nov;108(3):227-38
pubmed: 19008644
J Neurochem. 2003 Mar;84(6):1332-9
pubmed: 12614333
Neuropharmacology. 2016 Aug;107:376-386
pubmed: 27044663
Elife. 2014 Sep 17;3:e03970
pubmed: 25232658
EMBO J. 1997 Jul 1;16(13):3822-32
pubmed: 9233792
Front Cell Neurosci. 2015 Apr 08;9:119
pubmed: 25904845
J Neurosci. 2012 Apr 25;32(17):6000-13
pubmed: 22539860
Nucleic Acids Res. 2019 Jan 8;47(D1):D442-D450
pubmed: 30395289
J Neural Transm (Vienna). 2003 Dec;110(12):1337-48
pubmed: 14666406
J Clin Invest. 2011 Jan;121(1):446-53
pubmed: 21123949
J Neurosci. 2002 Dec 15;22(24):10643-52
pubmed: 12486157
Nat Rev Mol Cell Biol. 2011 Jul 22;12(8):517-33
pubmed: 21779028
J Cell Biol. 1995 Mar;128(6):1003-17
pubmed: 7896867
Neurochem Int. 2007 Nov-Dec;51(6-7):333-55
pubmed: 17517448
Annu Rev Biochem. 2009;78:857-902
pubmed: 19317650
Nat Rev Mol Cell Biol. 2012 Apr 13;13(5):323-8
pubmed: 22498832
Curr Opin Cell Biol. 2004 Aug;16(4):392-9
pubmed: 15261671
J Neurosci. 2014 Apr 30;34(18):6344-6351
pubmed: 24790205
J Biol Chem. 2006 May 12;281(19):13439-13448
pubmed: 16527819
Nature. 1992 Dec 3;360(6403):467-71
pubmed: 1280334
J Biol Chem. 2006 Feb 24;281(8):4876-86
pubmed: 16368696
J Neurosci. 2008 Nov 19;28(47):12453-64
pubmed: 19020038
J Biol Chem. 1998 Sep 18;273(38):24786-91
pubmed: 9733781
J Cell Biol. 2018 Sep 3;217(9):3161-3182
pubmed: 30061108
Nature. 2001 Mar 1;410(6824):84-8
pubmed: 11242046
Neuropharmacology. 2005 Nov;49(6):883-9
pubmed: 16212990
Neuron. 2014 Jul 16;83(2):404-416
pubmed: 25033183
J Cell Biol. 2000 Dec 11;151(6):1345-52
pubmed: 11121447
J Cell Sci. 2016 Aug 15;129(16):3104-14
pubmed: 27358480
J Biol Chem. 2008 Mar 7;283(10):6561-71
pubmed: 18096700
Neuron. 1994 Sep;13(3):713-25
pubmed: 7917301
Mol Biol Cell. 2004 Aug;15(8):3698-708
pubmed: 15155807
Nat Cell Biol. 2011 May;13(5):559-67
pubmed: 21499258
Nat Rev Mol Cell Biol. 2004 Feb;5(2):121-32
pubmed: 15040445
J Biol Chem. 2000 Feb 18;275(7):5228-37
pubmed: 10671571
J Cell Biol. 2001 Sep 3;154(5):1007-17
pubmed: 11535619
Nat Cell Biol. 2002 Dec;4(12):929-36
pubmed: 12447393
Nature. 2003 Mar 6;422(6927):37-44
pubmed: 12621426
Nat Neurosci. 2006 Jan;9(1):119-26
pubmed: 16311588
Traffic. 2010 Nov;11(11):1455-70
pubmed: 20727120
Neurochem Int. 2016 Sep;98:29-45
pubmed: 27235987
Nat Neurosci. 2009 May;12(5):568-76
pubmed: 19377470
J Neurochem. 2007 Dec;103(5):1917-31
pubmed: 17868307
Neurochem Int. 2016 Sep;98:4-18
pubmed: 27233497
J Comp Neurol. 2000 Mar 13;418(3):255-69
pubmed: 10701825
Nat Rev Mol Cell Biol. 2006 May;7(5):347-58
pubmed: 16633337
Science. 1997 Jun 13;276(5319):1699-702
pubmed: 9180080
Brain Res. 1999 Oct 16;845(1):92-6
pubmed: 10529447
Neurochem Int. 2014 Jul;73:152-8
pubmed: 24334055
J Biol Chem. 2019 Mar 8;294(10):3419-3431
pubmed: 30587577
J Biol Chem. 1994 Jan 14;269(2):1437-48
pubmed: 8288610
J Biol Chem. 2008 Mar 7;283(10):6175-83
pubmed: 18167356
J Neurosci. 2009 Nov 18;29(46):14581-95
pubmed: 19923291