Properties of spores of Bacillus subtilis with or without a transposon that decreases spore germination and increases spore wet heat resistance.
Bacillus
bacterial spores
food processing
food safety
spores
Journal
Journal of applied microbiology
ISSN: 1365-2672
Titre abrégé: J Appl Microbiol
Pays: England
ID NLM: 9706280
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
revised:
14
03
2021
received:
28
12
2020
accepted:
03
05
2021
pubmed:
28
5
2021
medline:
1
12
2021
entrez:
27
5
2021
Statut:
ppublish
Résumé
This work aimed to determine how genes on transposon Tn1546 slow Bacillus subtilis spore germination and increase wet heat resistance, and to clarify the transposon's 3 gene spoVA operon's role in spore properties, since the seven wild-type SpoVA proteins form a channel transporting Ca Deletion of the wild-type spoVA operon from a strain with Tn1546 gave spores with slightly reduced wet heat resistance but some large decreases in germination rate. Spore water content and CaDPA analyses found no significant differences in contents of either component in spores with different Tn1546 components or lacking the wild-type spoVA operon. This work indicates that the SpoVA channel encoded by Tn1546 functions like the wild-type SpoVA channel in CaDPA uptake into developing spores, but not as well in germination. The essentially identical CaDPA and water contents of spores with and without Tn1546 indicate that low core water content does not cause elevated wet heat resistance of spores with Tn1546. Since wet heat resistance of spores of Bacillus species poses problems in the food industry, understanding mechanisms of spores' wet heat resistance is of significant applied interest.
Substances chimiques
Bacterial Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2918-2928Subventions
Organisme : National Natural Science Foundation of China
ID : 61775036
Organisme : National Natural Science Foundation of China
ID : 91751110
Organisme : BaGui scholarship program of Guangxi Province, China
Informations de copyright
© 2021 The Society for Applied Microbiology.
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