Commentary on "Novel Interaction of the Dopamine D2 Receptor and the Ca
Journal
Molecular pharmacology
ISSN: 1521-0111
Titre abrégé: Mol Pharmacol
Pays: United States
ID NLM: 0035623
Informations de publication
Date de publication:
08 2021
08 2021
Historique:
received:
24
03
2021
accepted:
30
04
2021
pubmed:
29
5
2021
medline:
23
9
2021
entrez:
28
5
2021
Statut:
ppublish
Résumé
We previously proposed that the dopamine D2 receptor-interacting protein S100B binds to a putative S100B-binding motif at residues R233-L240 toward the N terminus of the third intracellular loop. We used in vitro pull-down assays with FLAG-tagged fragments of the rat dopamine D2 receptor third intracellular loop (D2-IC3) and in vitro-synthesized S100B to evaluate this hypothesis. Our results indicate that the putative S100B-binding motif is neither necessary nor sufficient for strong binding of S100B to D2-IC3. Instead, two residues at the junction of the fifth membrane-spanning domain and the cytoplasmic extension of that
Identifiants
pubmed: 34045267
pii: molpharm.121.000284
doi: 10.1124/molpharm.121.000284
pmc: PMC8382258
doi:
Substances chimiques
DRD2 protein, rat
0
Receptors, Dopamine D2
0
S100 Calcium Binding Protein beta Subunit
0
S100b protein, rat
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
61-64Subventions
Organisme : BLRD VA
ID : I01 BX000810
Pays : United States
Organisme : BLRD VA
ID : I01 BX003279
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH045372
Pays : United States
Informations de copyright
U.S. Government work not protected by U.S. copyright.
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