Red light stimulates vasodilation through extracellular vesicle trafficking.
Endothelium
Exocytosis
Extracellular vesicles
Nitric oxide
Photobiomodulation
Red light
S-nitrosothiol
Journal
Journal of photochemistry and photobiology. B, Biology
ISSN: 1873-2682
Titre abrégé: J Photochem Photobiol B
Pays: Switzerland
ID NLM: 8804966
Informations de publication
Date de publication:
Jul 2021
Jul 2021
Historique:
received:
18
12
2020
revised:
01
03
2021
accepted:
10
05
2021
pubmed:
29
5
2021
medline:
18
8
2021
entrez:
28
5
2021
Statut:
ppublish
Résumé
Red light (670 nm) promotes ex vivo dilation of blood vessels in a nitric oxide (NO) dependent, but eNOS independent manner by secreting a quasi-stable and transferable vasoactive substance with the characteristics of S-nitrosothiols (RSNO) from the endothelium. In the present work we establish that 670 nm light mediated vasodilation occurs in vivo and is physiologically stable. Light exposure depletes intracellular S-nitroso protein while concomitantly increasing extracellular RNSO, suggesting vesicular pathways are involved. Furthermore, we demonstrate this RSNO vasodilator is embedded in extracellular vesicles (EV). The action of red light on vesicular trafficking appears to increase expression of endosome associated membrane protein CD63 in bovine aortic endothelial cells, enhance endosome localization in the endothelium, and induce exit of RSNO containing EVs from murine facialis arteries. We suggest a mechanism by which the concerted actions of 670 nm light initiate formation of RSNO containing EVs which exit the endothelium and trigger relaxation of smooth muscle cells.
Identifiants
pubmed: 34049180
pii: S1011-1344(21)00091-9
doi: 10.1016/j.jphotobiol.2021.112212
pmc: PMC8240139
mid: NIHMS1708574
pii:
doi:
Substances chimiques
Nitroso Compounds
0
Sulfhydryl Compounds
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
112212Subventions
Organisme : BLRD VA
ID : I01 BX004675
Pays : United States
Organisme : BLRD VA
ID : IK2 BX002426
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL139557
Pays : United States
Informations de copyright
Published by Elsevier B.V.
Références
Ann Biomed Eng. 2012 Feb;40(2):516-33
pubmed: 22045511
Hypertension. 1990 Nov;16(5):477-83
pubmed: 1977698
Front Cell Dev Biol. 2017 Aug 30;5:78
pubmed: 28913335
Nitric Oxide. 2018 May 1;75:60-69
pubmed: 29428841
Eur J Appl Physiol. 2018 May;118(5):1043-1052
pubmed: 29516257
J Cell Biochem. 2006 Apr 15;97(6):1393-406
pubmed: 16365884
Hypertension. 1996 Sep;28(3):354-60
pubmed: 8794816
Am J Physiol Lung Cell Mol Physiol. 2018 Jan 1;314(1):L93-L106
pubmed: 28882814
Nanomedicine. 2011 Dec;7(6):780-8
pubmed: 21601655
J Pharmacol Exp Ther. 1989 Feb;248(2):687-95
pubmed: 2537410
Nitric Oxide. 2020 Jan 1;94:108-113
pubmed: 31759127
Br J Pharmacol. 2003 Mar;138(5):932-40
pubmed: 12642395
IEEE J Sel Top Quantum Electron. 2016 May-Jun;22(3):
pubmed: 28070154
J Extracell Vesicles. 2012 Apr 16;1:
pubmed: 24009886
J Cell Mol Med. 2016 Aug;20(8):1457-66
pubmed: 26999719
Arch Biochem Biophys. 2018 Jul 1;649:47-52
pubmed: 29752896
J Mol Cell Cardiol. 2013 Sep;62:36-42
pubmed: 23702287
Proc Natl Acad Sci U S A. 2014 Dec 16;111(50):17977-82
pubmed: 25404319
Chem Res Toxicol. 2012 May 21;25(5):975-89
pubmed: 22449080
J Mol Cell Cardiol. 2009 Aug;47(2):256-63
pubmed: 19328206
Semin Cell Dev Biol. 2017 Jul;67:48-55
pubmed: 28104520
BMC Biol. 2012 Jan 30;10:4
pubmed: 22289422
Arch Surg. 1981 Mar;116(3):293-6
pubmed: 7469766
Front Physiol. 2017 May 05;8:275
pubmed: 28529488
Free Radic Biol Med. 2017 Dec;113:157-164
pubmed: 28935419
J Photochem Photobiol B. 1999 Mar;49(1):1-17
pubmed: 10365442
Environ Mol Mutagen. 2018 Jun;59(5):438-460
pubmed: 29466611
Nat Rev Mol Cell Biol. 2013 May;14(5):283-96
pubmed: 23609508
Exp Cell Res. 2009 May 15;315(9):1584-92
pubmed: 18930046
Mol Biol Cell. 2000 May;11(5):1829-43
pubmed: 10793155
Histochem Cell Biol. 2020 Jun;153(6):431-441
pubmed: 32162135
J Gen Physiol. 1961 Jan;44:499-519
pubmed: 13702637
Redox Biol. 2019 Jan;20:13-18
pubmed: 30261342