Live Imaging of Virus-Infected Cells by Using a Sialidase Fluorogenic Probe.
Drug resistance
Fluorescence imaging
Imaging probe
Infected cells
Influenza virus
Neuraminidase
Paramyxovirus
Sialidase
Virus isolation
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
29
5
2021
pubmed:
30
5
2021
medline:
22
6
2021
Statut:
ppublish
Résumé
Visualization of virus-infected cells is usually performed by immunostaining with an antiviral antibody. On the other hand, we established an easy method for fluorescence (FL) imaging of cells infected with influenza A and B viruses and some paramyxoviruses without the need for cell fixation and an antiviral antibody. These viruses and the cells they have infected express the viral surface enzyme "neuraminidase" or "hemagglutinin-neuraminidase" that shows sialidase activity. Sialidase activity is fluorescently visualized by using a sialidase fluorogenic probe developed in our previous study. The probe enables histochemical FL imaging of the virus-infected cells and is applicable to virus isolation and detection of an influenza virus resistant to antiinfluenza drugs of sialidase inhibitors.
Identifiants
pubmed: 34050469
doi: 10.1007/978-1-0716-1258-3_13
doi:
Substances chimiques
Antiviral Agents
0
Neuraminidase
EC 3.2.1.18
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
141-154Références
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