Bioluminescence Imaging of Neuronal Network Dynamics Using Aequorin-Based Calcium Sensors.
Bioluminescence
Calcium imaging
Coelenterazine (CTZ)
GFP-aequorin
Genetically encoded probe
Neuron
Neuronal ensemble
Viral transfer
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
29
5
2021
pubmed:
30
5
2021
medline:
22
6
2021
Statut:
ppublish
Résumé
Optogenetic calcium sensors enable the imaging in real-time of the activities of single or multiple neurons in brain slices and in vivo. Bioluminescent probes engineered from the natural calcium sensor aequorin do not require illumination, are virtually devoid of background signal, and exhibit wide dynamic range and low cytotoxicity. These probes are thus well suited for long-duration, whole-field recordings of multiple neurons simultaneously. Here, we describe a protocol for monitoring and analyzing the dynamics of neuronal ensembles using whole-field bioluminescence imaging of an aequorin-based sensor in brain slice.
Identifiants
pubmed: 34050480
doi: 10.1007/978-1-0716-1258-3_24
doi:
Substances chimiques
Luminescent Agents
0
Aequorin
50934-79-7
Calcium
SY7Q814VUP
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
281-294Références
Ni Q, Mehta S, Zhang J (2018) Live-cell imaging of cell signaling using genetically encoded fluorescent reporters. FEBS J 285(2):203–219. https://doi.org/10.1111/febs.14134
doi: 10.1111/febs.14134
pubmed: 28613457
Perez Koldenkova V, Nagai T (2013) Genetically encoded Ca
doi: 10.1016/j.bbamcr.2013.01.011
pubmed: 23352808
Wilson T, Hastings JW (1998) Bioluminescence. Annu Rev Cell Dev Biol 14:197–230. https://doi.org/10.1146/annurev.cellbio.14.1.197
doi: 10.1146/annurev.cellbio.14.1.197
pubmed: 9891783
Pinton P, Rimessi A, Romagnoli A, Prandini A, Rizzuto R (2007) Biosensors for the detection of calcium and pH. Methods Cell Biol 80:297–325. https://doi.org/10.1016/S0091-679X(06)80015-4
doi: 10.1016/S0091-679X(06)80015-4
pubmed: 17445701
Vysotski ES, Lee J (2004) Ca
doi: 10.1021/ar0400037
pubmed: 15196050
Hastings JW, Mitchell G, Mattingly PH, Blinks JR, Van Leeuwen M (1969) Response of aequorin bioluminescence to rapid changes in calcium concentration. Nature 222(5198):1047–1050
doi: 10.1038/2221047a0
Shimomura O, Johnson FH (1970) Calcium binding, quantum yield, and emitting molecule in aequorin bioluminescence. Nature 227(5265):1356. https://doi.org/10.1038/2271356a0
doi: 10.1038/2271356a0
pubmed: 4393938
Shimomura O, Johnson FH (1975) Regeneration of the photoprotein aequorin. Nature 256(5514):236–238
doi: 10.1038/256236a0
Tricoire L, Tsuzuki K, Courjean O, Gibelin N, Bourout G, Rossier J, Lambolez B (2006) Calcium dependence of aequorin bioluminescence dissected by random mutagenesis. Proc Natl Acad Sci U S A 103(25):9500–9505. https://doi.org/10.1073/pnas.0603176103
doi: 10.1073/pnas.0603176103
pubmed: 16769886
pmcid: 1480436
Agulhon C, Platel J-C, Kolomiets B, Forster V, Picaud S, Brocard J, Faure P, Brulet P (2007) Bioluminescent imaging of Ca
doi: 10.1113/jphysiol.2007.135715
pubmed: 17627996
pmcid: 2277207
Brini M, Pinton P, Pozzan T, Rizzuto R (1999) Targeted recombinant aequorins: tools for monitoring [Ca
doi: 10.1002/(SICI)1097-0029(19990915)46:6<380::AID-JEMT6>3.0.CO;2-Y
pubmed: 10504215
Drobac E, Tricoire L, Chaffotte AF, Guiot E, Lambolez B (2010) Calcium imaging in single neurons from brain slices using bioluminescent reporters. J Neurosci Res 88(4):695–711. https://doi.org/10.1002/jnr.22249
doi: 10.1002/jnr.22249
pubmed: 19798746
Dubuisson MLN, de Wergifosse B, Trouet A, Baguet F, Marchand-Brynaert J, Rees JF (2000) Antioxidative properties of natural coelenterazine and synthetic methyl coelenterazine in rat hepatocytes subjected to tert-butyl hydroperoxide-induced oxidative stress. Biochem Pharmacol 60(4):471–478. https://doi.org/10.1016/s0006-2952(00)00359-2
doi: 10.1016/s0006-2952(00)00359-2
pubmed: 10874121
Shakhmin A, Hall MP, Walker JR, Machleidt T, Binkowski BF, Wood KV, Kirkland TA (2016) Three efficient methods for preparation of Coelenterazine analogues. Chem Eur J 22(30):10369–10375. https://doi.org/10.1002/chem.201601111
doi: 10.1002/chem.201601111
pubmed: 27305599
Rogers KL, Picaud S, Roncali E, Boisgard R, Colasante C, Stinnakre J, Tavitian B, Brulet P (2007) NonInvasive in vivo imaging of calcium signaling in mice. PLoS One 2(10). https://doi.org/10.1371/journal.pone.0000974
Rogers KL, Stinnakre J, Agulhon C, Jublot D, Shorte SL, Kremer EJ, Brulet P (2005) Visualization of local Ca
doi: 10.1111/j.1460-9568.2005.03871.x
pubmed: 15733079
Tricoire L, Drobac E, Tsuzuki K, Gallopin T, Picaud S, Cauli B, Rossier J, Lambolez B (2019) Bioluminescence calcium imaging of network dynamics and their cholinergic modulation in slices of cerebral cortex from male rats. J Neurosci Res 97(4):414–432. https://doi.org/10.1002/jnr.24380
doi: 10.1002/jnr.24380
pubmed: 30604494
Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, Yoshida Y, Matsuda T, Arai Y, Nagai T (2012) Luminescent proteins for high-speed single-cell and whole-body imaging. Nat Commun 3:1262. https://doi.org/10.1038/ncomms2248
doi: 10.1038/ncomms2248
pubmed: 23232392
Suzuki K, Kimura T, Shinoda H, Bai G, Daniels MJ, Arai Y, Nakano M, Nagai T (2016) Five-colour variants of bright luminescent protein for real-time multicolour bioimaging. Nat Commun 7. https://doi.org/10.1038/ncomms13718
Allen DG, Blinks JR, Prendergast FG (1977) Aequorin luminescence: relation of light emission to calcium concentration--a calcium-independent component. Science 195(4282):996–998
doi: 10.1126/science.841325
Baubet V, Le Mouellic H, Campbell AK, Lucas-Meunier E, Fossier P, Brulet P (2000) Chimeric green fluorescent protein-aequorin as bioluminescent Ca
doi: 10.1073/pnas.97.13.7260
pubmed: 10860991
pmcid: 16533
Knafo S, Prendergast A, Thouvenin O, Figueiredo SN, Wyart C (2017) Bioluminescence monitoring of neuronal activity in freely moving zebrafish larvae. Bio-Protocol 7(18). https://doi.org/10.21769/BioProtoc.2550
Martin JR, Rogers KL, Chagneau C, Brulet P (2007) In vivo bioluminescence imaging of ca signaling in the brain of drosophila. PLoS One 2(3):e275. https://doi.org/10.1371/journal.pone.0000275
doi: 10.1371/journal.pone.0000275
pubmed: 17342209
pmcid: 1803028
Naumann EA, Kampff AR, Prober DA, Schier AF, Engert F (2010) Monitoring neural activity with bioluminescence during natural behavior. Nat Neurosci 13(4):U513–U146. https://doi.org/10.1038/nn.2518
doi: 10.1038/nn.2518
Morin JG, Hastings JW (1971) Energy transfer in a bioluminescent system. J Cell Physiol 77(3):313–318. https://doi.org/10.1002/jcp.1040770305
doi: 10.1002/jcp.1040770305
pubmed: 4397528
Bakayan A, Vaquero CF, Picazo F, Llopis J (2011) Red fluorescent protein-Aequorin fusions as improved bioluminescent Ca
Curie T, Rogers KL, Colasante C, Brulet P (2007) Red-shifted aequorin-based bioluminescent reporters for in vivo imaging of Ca
doi: 10.2310/7290.2006.00033
Tricoire L, Lambolez B (2014) Neuronal network imaging in acute slices using Ca
doi: 10.1007/978-1-62703-718-1_3
Tricoire L, Drobac E, Lambolez B (2012) Ca
doi: 10.1007/978-1-62703-014-4
Altman-Hamamdzic S, Groseclose C, Ma JX, Hamamdzic D, Vrindavanam NS, Middaugh LD, Parratto NP, Sallee FR (1997) Expression of beta-galactosidase in mouse brain: utilization of a novel nonreplicative Sindbis virus vector as a neuronal gene delivery system. Gene Ther 4(8):815–822. https://doi.org/10.1038/sj.gt.3300458
doi: 10.1038/sj.gt.3300458
pubmed: 9338010
Gwag BJ, Kim EY, Ryu BR, Won SJ, Ko HW, Oh YJ, Cho YG, Ha SJ, Sung YC (1998) A neuron-specific gene transfer by a recombinant defective Sindbis virus. Brain Res Mol Brain Res 63(1):53–61
doi: 10.1016/S0169-328X(98)00251-4
Hepp R, Tricoire L, Hu E, Gervasi N, Paupardin-Tritsch D, Lambolez B, Vincent P (2007) Phosphodiesterase type 2 and the homeostasis of cyclic GMP in living thalamic neurons. J Neurochem 102(6):1875–1886. https://doi.org/10.1111/j.1471-4159.2007.04657.x
doi: 10.1111/j.1471-4159.2007.04657.x
pubmed: 17561940
Ehrengruber MU, Lundstrom K (2007) Alphaviruses: Semliki Forest virus and Sindbis virus vectors for gene transfer into neurons. Curr Protoc Neurosci. Chapter 4:Unit 4 22. https://doi.org/10.1002/0471142301.ns0422s41