European multicenter evaluation of Xpert® Xpress SARS-CoV-2/Flu/RSV test.
COVID-19
/ diagnosis
COVID-19 Nucleic Acid Testing
Europe
/ epidemiology
Humans
Influenza A virus
/ isolation & purification
Influenza B virus
/ isolation & purification
Influenza, Human
/ diagnosis
Molecular Diagnostic Techniques
Multiplex Polymerase Chain Reaction
Nasopharynx
/ virology
Respiratory Syncytial Virus Infections
/ diagnosis
Respiratory Syncytial Virus, Human
/ isolation & purification
Respiratory Tract Infections
/ diagnosis
SARS-CoV-2
/ genetics
Sensitivity and Specificity
COVID-19
SARS-CoV-2
influenza
multiplex PCR
pandemic
respiratory syncytial virus
Journal
Journal of medical virology
ISSN: 1096-9071
Titre abrégé: J Med Virol
Pays: United States
ID NLM: 7705876
Informations de publication
Date de publication:
Oct 2021
Oct 2021
Historique:
revised:
07
05
2021
received:
25
03
2021
accepted:
27
05
2021
pubmed:
30
5
2021
medline:
20
8
2021
entrez:
29
5
2021
Statut:
ppublish
Résumé
Rapid diagnostics for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are paramount for reducing the spread of the current pandemic. During additional seasonal epidemics with influenza A/B and respiratory syncytial virus (RSV), the clinical signs and symptoms cannot be distinguished easily from SARS-CoV-2. Therefore, a new assay combining four targets in the form of the new Xpert Xpress SARS-CoV-2/Flu/RSV assay was evaluated. The assay was compared to the Xpert Xpress SARS-CoV-2, Xpert Xpress Flu/RSV, Seegene Flu/RSV, influenza A/B r-gene® and RSV/hMPV r-gene®. A total of 295 nasopharyngeal and throat swabs were tested at four institutes throughout Europe including 72 samples positive for SARS-CoV-2, 65 for influenza A, 47 for influenza B, and 77 for RSV. The sensitivity of the new assay was above 95% for all targets, with the highest for SARS-CoV-2 (97.2%). The overall correlation of SARS-CoV-2 Ct values between Xpert Xpress SARS-CoV-2 assay and Xpert Xpress SARS-CoV-2/Flu/RSV assay was high. The agreement between Ct values above 30 showed the multiplex giving higher Ct values for SARS-CoV-2 on average than the singleplex assay. In conclusion, the new assay is a rapid and reliable alternative with less hands-on time for the detection of not one, but four upper respiratory tract pathogens that may circulate at the same time.
Identifiants
pubmed: 34050951
doi: 10.1002/jmv.27111
pmc: PMC8242864
doi:
Types de publication
Evaluation Study
Journal Article
Multicenter Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
5798-5804Informations de copyright
© 2021 The Authors. Journal of Medical Virology published by Wiley Periodicals LLC.
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