Optimal sample size for calibrating DNA methylation age estimators.
DNA methylation
age estimation
epigenetic clock
sample size
wildlife
Journal
Molecular ecology resources
ISSN: 1755-0998
Titre abrégé: Mol Ecol Resour
Pays: England
ID NLM: 101465604
Informations de publication
Date de publication:
Oct 2021
Oct 2021
Historique:
revised:
13
05
2021
received:
28
09
2020
accepted:
25
05
2021
pubmed:
31
5
2021
medline:
18
9
2021
entrez:
30
5
2021
Statut:
ppublish
Résumé
Age is a fundamental parameter in wildlife management as it is used to determine the risk of extinction, manage invasive species, and regulate sustainable harvest. In a broad variety of vertebrates species, age can be determined by measuring DNA methylation. Animals with known ages are initially required during development, calibration, and validation of these epigenetic clocks. However, wild animals with known ages are frequently difficult to obtain. Here, we perform Monte-Carlo simulations to determine the optimal sample size required to create an accurate calibration model for age estimation by elastic net regression modelling of cytosine-phosphate-guanine methylation data. Our results suggest a minimum calibration population size of 70, but ideally 134 individuals or more for accurate and precise models. We also provide estimates to the extent a model can be extrapolated beyond a distribution of ages that was used during calibration. The findings can assist researchers to better design age estimation models and decide if their model is adequate for determining key population attributes.
Identifiants
pubmed: 34053192
doi: 10.1111/1755-0998.13437
pmc: PMC8518423
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2316-2323Subventions
Organisme : CSIRO
Informations de copyright
© 2021 The Authors. Molecular Ecology Resources published by John Wiley & Sons Ltd.
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