Patient Derived Colonoids as Drug Testing Platforms-Critical Importance of Oxygen Concentration.
cytokines
epithelium
inflammatory bowel diseases
interleukin 17
organoids
oxygen
tumor necrosis factor
Journal
Frontiers in pharmacology
ISSN: 1663-9812
Titre abrégé: Front Pharmacol
Pays: Switzerland
ID NLM: 101548923
Informations de publication
Date de publication:
2021
2021
Historique:
received:
12
03
2021
accepted:
28
04
2021
entrez:
31
5
2021
pubmed:
1
6
2021
medline:
1
6
2021
Statut:
epublish
Résumé
Treatment of inflammatory bowel disease (IBD) is challenging, with a series of available drugs each helping only a fraction of patients. Patients may face time-consuming drug trials while the disease is active, thus there is an unmet need for biomarkers and assays to predict drug effect. It is well known that the intestinal epithelium is an important factor in disease pathogenesis, exhibiting physical, biochemical and immunologic driven barrier dysfunctions. One promising test system to study effects of existing or emerging IBD treatments targeting intestinal epithelial cells (IECs) is intestinal organoids ("mini-guts"). However, the fact that healthy intestinal epithelium is in a physiologically hypoxic state has largely been neglected, and studies with intestinal organoids are mainly performed at oxygen concentration of 20%. We hypothesized that lowering the incubator oxygen level from 20% to 2% would recapitulate better the
Identifiants
pubmed: 34054553
doi: 10.3389/fphar.2021.679741
pii: 679741
pmc: PMC8156408
doi:
Types de publication
Journal Article
Langues
eng
Pagination
679741Informations de copyright
Copyright © 2021 Skovdahl, Gopalakrishnan, Svendsen, Granlund, Bakke, Ginbot, Thorsvik, Flatberg, Sporsheim, Ostrop, Mollnes, Sandvik and Bruland.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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