Extended Archaeal Histone-Based Chromatin Structure Regulates Global Gene Expression in

Histone proteins compact and organize DNA resulting in a dynamic chromatin architecture impacting DNA accessibility and ultimately gene expression. Eukaryotic chromatin landscapes are structured through histone protein variants, epigenetic marks, the activities of chromatin-remodeling complexes, and post-translational modification of histone proteins. In most Archaea, histone-based chromatin structure is dominated by the helical polymerization of histone proteins wrapping DNA into a repetitive and closely gyred configuration. The formation of the archaeal-histone chromatin-superhelix is a regulatory force of adaptive gene expression and is likely critical for regulation of gene expression in all histone-encoding Archaea. Single amino acid substitutions in archaeal histones that block formation of tightly packed chromatin structures have profound effects on cellular fitness, but the underlying gene expression changes resultant from an altered chromatin landscape have not been resolved. Using the model organism

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Copyright © 2021 Sanders, Ullah, Gehring, Burkhart, Vickerman, Fernando, Gardner, Ben-Hur and Santangelo.

Déclaration de conflit d'intérêts

AGe and AGa are employed and funded by New England Biolabs, Inc. New England Biolabs is a manufacturer and vendor of molecular biology reagents, including DNA replication and repair enzymes. This affiliation does not affect the authors’ impartiality, objectivity of data generation or its interpretation, adherence to journal standards and policies or availability of data. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.