Urothelial Cancer Associated 1 (UCA1) and miR-193 Are Two Non-coding RNAs Involved in Trophoblast Fusion and Placental Diseases.
intra uterine growth restriction
non-coding RNAs
placenta
preeclampsia
syncytialisation
trophoblast
Journal
Frontiers in cell and developmental biology
ISSN: 2296-634X
Titre abrégé: Front Cell Dev Biol
Pays: Switzerland
ID NLM: 101630250
Informations de publication
Date de publication:
2021
2021
Historique:
received:
26
11
2020
accepted:
19
04
2021
entrez:
31
5
2021
pubmed:
1
6
2021
medline:
1
6
2021
Statut:
epublish
Résumé
A bioinformatics screen for non-coding genes was performed from microarrays analyzing on the one hand trophoblast fusion in the BeWo cell model, and on the other hand, placental diseases (preeclampsia and Intra-Uterine Growth Restriction). Intersecting the deregulated genes allowed to identify two miRNA (mir193b and miR365a) and one long non-coding RNA (UCA1) that are pivotal for trophoblast fusion, and deregulated in placental diseases. We show that miR-193b is a hub for the down-regulation of 135 cell targets mainly involved in cell cycle progression and energy usage/nutrient transport. UCA1 was explored by siRNA knock-down in the BeWo cell model. We show that its down-regulation is associated with the deregulation of important trophoblast physiology genes, involved in differentiation, proliferation, oxidative stress, vacuolization, membrane repair and endocrine production. Overall, UCA1 knockdown leads to an incomplete gene expression profile modification of trophoblast cells when they are induced to fuse into syncytiotrophoblast. Then we performed the same type of analysis in cells overexpressing one of the two major isoforms of the STOX1 transcription factor, STOX1A and STOX1B (associated previously to impaired trophoblast fusion). We could show that when STOX1B is abundant, the effects of UCA1 down-regulation on forskolin response are alleviated.
Identifiants
pubmed: 34055770
doi: 10.3389/fcell.2021.633937
pmc: PMC8155540
doi:
Types de publication
Journal Article
Langues
eng
Pagination
633937Informations de copyright
Copyright © 2021 Apicella, Ruano, Jacques, Gascoin, Méhats, Vaiman and Miralles.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Am J Pathol. 2013 Jul;183(1):144-52
pubmed: 23680657
Reproduction. 2010 Nov;140(5):759-66
pubmed: 20696850
BMJ. 2018 Oct 17;363:k4109
pubmed: 30333106
Ann N Y Acad Sci. 2008 Apr;1127:129-33
pubmed: 18443340
Epigenetics. 2013 Feb;8(2):192-202
pubmed: 23314690
Genomics. 2020 Jan;112(1):574-580
pubmed: 30998966
Tumour Biol. 2014 Jul;35(7):6901-12
pubmed: 24737584
Hum Genet. 2021 May;140(5):827-848
pubmed: 33433680
Placenta. 2020 Mar;92:17-27
pubmed: 32056783
BJOG. 2018 Dec;125(13):1642-1654
pubmed: 29978553
Mol Cancer. 2019 Jul 4;18(1):115
pubmed: 31272462
J Cancer Res Clin Oncol. 2016 Jul;142(7):1407-19
pubmed: 26341664
Rev Bras Ginecol Obstet. 2017 Jul;39(7):315-316
pubmed: 28605820
Expert Rev Cardiovasc Ther. 2007 Mar;5(2):283-94
pubmed: 17338672
Reproduction. 2018 Jun;155(6):R259-R271
pubmed: 29615475
Hypertension. 2012 Feb;59(2):265-73
pubmed: 22203747
BMC Genomics. 2017 Jul 10;18(1):520
pubmed: 28693416
Sci Rep. 2016 Jan 13;6:19196
pubmed: 26758611
J Reprod Med. 2008 Aug;53(8):608-14
pubmed: 18773626
Nucleic Acids Res. 2019 Jul 2;47(W1):W199-W205
pubmed: 31114916
Best Pract Res Clin Endocrinol Metab. 2019 Jun;33(3):101260
pubmed: 30709755
Placenta. 2015 Dec;36(12):1342-51
pubmed: 26515927
iScience. 2020 May 22;23(5):101086
pubmed: 32371375
Genes Genomics. 2019 Jul;41(7):803-810
pubmed: 30877641
Hypertension. 2013 Mar;61(3):561-3
pubmed: 23357180
Am J Obstet Gynecol. 2017 Aug;217(2):200.e1-200.e17
pubmed: 28347715
Placenta. 2009 Mar;30 Suppl A:S49-54
pubmed: 19027159
Cancers (Basel). 2018 Nov 14;10(11):
pubmed: 30441811
Clin Cancer Res. 2006 Aug 15;12(16):4851-8
pubmed: 16914571
Am J Obstet Gynecol. 2015 Oct;213(4 Suppl):S138-51
pubmed: 26428493
Biol Reprod. 2014 Jun;90(6):117
pubmed: 24740598
Cell Mol Life Sci. 2016 Jan;73(2):365-76
pubmed: 26496726
Cell Rep. 2017 Oct 31;21(5):1150-1159
pubmed: 29091755
J Cell Physiol. 1986 Sep;128(3):375-82
pubmed: 3745281
Eur Rev Med Pharmacol Sci. 2018 Nov;22(21):7274-7281
pubmed: 30468471
J Clin Invest. 2012 Nov;122(11):4003-11
pubmed: 23093777
Genome Res. 2003 Nov;13(11):2498-504
pubmed: 14597658
BMC Med Genomics. 2019 Jun 27;12(1):91
pubmed: 31248403
Reproduction. 2016 Oct 14;:
pubmed: 27742864
Eur Rev Med Pharmacol Sci. 2019 Nov;23(21):9238-9250
pubmed: 31773675
Cell Death Dis. 2013 Apr 18;4:e593
pubmed: 23598405
Am J Reprod Immunol. 2010 Sep;64(3):159-69
pubmed: 20367628
J Clin Oncol. 2018 Apr 1;36(10):1007-1016
pubmed: 29432078
Circ Res. 2019 Mar 29;124(7):1094-1112
pubmed: 30920918
Reprod Sci. 2011 Mar;18(3):219-28
pubmed: 21135203
Pflugers Arch. 2019 Feb;471(2):347-355
pubmed: 30353369
Sci Rep. 2016 Jan 29;6:19910
pubmed: 26822621
Mol Cell Endocrinol. 2016 Jan 15;420:180-93
pubmed: 26586208
Development. 2019 Nov 27;146(22):
pubmed: 31776138
Adv Exp Med Biol. 2016;937:3-17
pubmed: 27573892
Biomed Pharmacother. 2017 Dec;96:1275-1282
pubmed: 29169729
Placenta. 2008 Jun;29(6):492-502
pubmed: 18420268
Cancer Res. 2017 Nov 1;77(21):5728-5740
pubmed: 28882999
J Cell Physiol. 2020 Oct;235(10):7410-7419
pubmed: 32067230
Int J Mol Sci. 2019 Mar 26;20(6):
pubmed: 30917529
Int J Mol Sci. 2019 Jun 11;20(11):
pubmed: 31212604
Expert Opin Ther Targets. 2016 Dec;20(12):1433-1443
pubmed: 27782763
Onco Targets Ther. 2018 May 01;11:2475-2487
pubmed: 29760557
Lancet. 2010 Aug 21;376(9741):631-44
pubmed: 20598363
Hypertension. 2014 Jun;63(6):1276-84
pubmed: 24664294
Placenta. 2004 Jul;25(6):479-88
pubmed: 15135230
Front Genet. 2020 Jan 09;10:1292
pubmed: 31998361
Am J Obstet Gynecol. 2019 Jan;220(1):110.e1-110.e21
pubmed: 30312585
Int J Mol Sci. 2020 May 20;21(10):
pubmed: 32443673
Lancet. 2008 Jan 5;371(9606):75-84
pubmed: 18177778
Noncoding RNA. 2019 Feb 17;5(1):
pubmed: 30781588