Expression and Localization of AβPP in SH-SY5Y Cells Depends on Differentiation State.
Alzheimer Disease
/ metabolism
Amyloid beta-Protein Precursor
/ metabolism
Brain-Derived Neurotrophic Factor
/ metabolism
Cell Differentiation
/ physiology
Cell Line, Tumor
Humans
Intravital Microscopy
/ methods
Models, Biological
Neuroblastoma
Neurons
/ physiology
Oxidative Stress
Proteolysis
Tretinoin
/ metabolism
Alzheimer’s disease
AβPP
SH-SY5Y
differentiation
super-resolution microscopy
Journal
Journal of Alzheimer's disease : JAD
ISSN: 1875-8908
Titre abrégé: J Alzheimers Dis
Pays: Netherlands
ID NLM: 9814863
Informations de publication
Date de publication:
2021
2021
Historique:
pubmed:
1
6
2021
medline:
21
9
2021
entrez:
31
5
2021
Statut:
ppublish
Résumé
Neuroblastoma cell line SH-SY5Y, due to its capacity to differentiate into neurons, easy handling, and low cost, is a common experimental model to study molecular events leading to Alzheimer's disease (AD). However, it is prevalently used in its undifferentiated state, which does not resemble neurons affected by the disease. Here, we show that the expression and localization of amyloid-β protein precursor (AβPP), one of the key molecules involved in AD pathogenesis, is dramatically altered in SH-SY5Y cells fully differentiated by combined treatment with retinoic acid and BDNF. We show that insufficient differentiation of SH-SY5Y cells results in AβPP mislocalization.
Identifiants
pubmed: 34057078
pii: JAD201409
doi: 10.3233/JAD-201409
pmc: PMC8385523
doi:
Substances chimiques
Amyloid beta-Protein Precursor
0
Brain-Derived Neurotrophic Factor
0
Tretinoin
5688UTC01R
Types de publication
Letter
Langues
eng
Sous-ensembles de citation
IM
Pagination
485-491Références
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