3D Engineering of Human Hematopoietic Niches in Perfusion Bioreactor.
3D culture
Bone marrow organoid
Hematopoietic niche
Human hematopoiesis
Mesenchymal stromal cells
Perfusion bioreactor
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
31
5
2021
pubmed:
1
6
2021
medline:
11
8
2021
Statut:
ppublish
Résumé
The hematopoietic microenvironment, also referred to as hematopoietic niche, is a functional three-dimensional (3D) unit of the bone marrow (BM) that planar culture systems cannot recapitulate. Existing limitations of 2D protocols are driving the development of advanced 3D methodologies, capable of superior modeling of the native organization and interactions between hematopoietic cells and their niche.Hereafter we describe the use of a 3D perfusion bioreactor for in vitro generation of human hematopoietic niches. The approach enables the recapitulation of the interactions between hematopoietic stem and progenitor cells (HSPCs), mesenchymal cells (MSCs), and their extracellular matrix in a 3D relevant setting. This was shown to support the functional maintenance of blood populations, self-distributing in the system compartments depending on their differentiation status. Such 3D niche modeling represents an advanced tool toward uncovering human hematopoiesis in relation to its host microenvironment , for both fundamental hematopoiesis and personalized medicine applications.
Identifiants
pubmed: 34057728
doi: 10.1007/978-1-0716-1425-9_19
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
253-262Références
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