Assessment of Mitochondrial Cell Metabolism by Respiratory Chain Electron Flow Assays.
Bioinformatics
Cancer metabolism
Electron transport chain
Mitochondrial respiration
Tricarboxylic acid cycle
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
1
6
2021
pubmed:
2
6
2021
medline:
9
7
2021
Statut:
ppublish
Résumé
Cellular energy metabolism is regulated by complex metabolic pathways. Although anaerobic glycolysis was reported as a primary source of energy in cancer leading to a high rate of lactate production, current evidence shows that the main energy source supporting cancer cell metabolism relies on mitochondrial metabolism. Mitochondria are the key organelle maintaining optimal cellular energy levels. MitoPlate™ S-1 provides a highly reproducible bioenergetics tool to analyze the electron flow rate in live cells. Measuring the rates of electron flow into and through the electron transport chain using different NADH and FADH
Identifiants
pubmed: 34060037
doi: 10.1007/978-1-0716-1266-8_9
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
129-141Références
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