Analysis of Mitochondrial Dysfunction During Cell Death.
Cell death
Membrane potential
Mitochondria
Reactive oxygen species
Respiration
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
1
6
2021
pubmed:
2
6
2021
medline:
9
7
2021
Statut:
ppublish
Résumé
Mitochondria play a key role in various modes of cell death. Analysis of mitochondrial dysfunction and the release of proteins from the intermembrane space of mitochondria represent essential tools in cell death investigation. Here we describe how to evaluate release of intermembrane space proteins during apoptosis, alterations in the mitochondrial membrane potential, and oxygen consumption in apoptotic cells.
Identifiants
pubmed: 34060044
doi: 10.1007/978-1-0716-1266-8_16
doi:
Substances chimiques
Cytochromes c
9007-43-6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
215-225Références
Galluzzi L, Vitale I, Aaronson SA et al (2018) Molecular mechanisms of cell death: recommendations of the nomenclature committee on cell death 2018. Cell Death Differ 25(3):486–541
doi: 10.1038/s41418-017-0012-4
Kerr JF, Wyllie AH, Currie AR (1972) Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer 26(4):239–257
doi: 10.1038/bjc.1972.33
Lowe SW, Lin AW (2000) Apoptosis in cancer. Carcinogenesis 21(3):485–495
doi: 10.1093/carcin/21.3.485
Gogvadze V, Orrenius S, Zhivotovsky B (2006) Multiple pathways of cytochrome c release from mitochondria in apoptosis. Biochim Biophys Acta 1757(5–6):639–647
doi: 10.1016/j.bbabio.2006.03.016
Zou H, Li Y, Liu X, Wang X (1999) An APAF-1.Cytochrome c multimeric complex is a functional apoptosome that activates procaspase-9. J Biol Chem 274(17):11549–11556
doi: 10.1074/jbc.274.17.11549
Robertson JD, Orrenius S, Zhivotovsky B (2000) Review: nuclear events in apoptosis. J Struct Biol 129(2–3):346–358
doi: 10.1006/jsbi.2000.4254
Crompton M (1999) The mitochondrial permeability transition pore and its role in cell death. Biochem J 341(Pt 2):233–249
doi: 10.1042/bj3410233
Martinou JC, Green DR (2001) Breaking the mitochondrial barrier. Nat Rev Mol Cell Biol 2(1):63–67
doi: 10.1038/35048069
Gogvadze V, Robertson JD, Zhivotovsky B, Orrenius S (2001) Cytochrome c release occurs via Ca
doi: 10.1074/jbc.M100614200
Mooney RA (1988) Use of digitonin permeabilized adipocytes for cAMP studies. Methods Enzymol 159:193–202
doi: 10.1016/0076-6879(88)59020-1
Mitchell P, Moyle J (1967) Chemiosmotic hypothesis of oxidative phosphorylation. Nature 213(5072):137–139
doi: 10.1038/213137a0
Carafoli E, Crompton M (1978) The regulation of intracellular calcium by mitochondria. Ann N Y Acad Sci 307:269–284
doi: 10.1111/j.1749-6632.1978.tb41957.x
Zamzami N, Susin SA, Marchetti P, Hirsch T, Gomez-Monterrey I, Castedo M, Kroemer G (1996) Mitochondrial control of nuclear apoptosis. J Exp Med 183(4):1533–1544
doi: 10.1084/jem.183.4.1533
Bossy-Wetzel E, Newmeyer DD, Green DR (1998) Mitochondrial cytochrome c release in apoptosis occurs upstream of DEVD-specific caspase activation and independently of mitochondrial transmembrane depolarization. EMBO J 17(1):37–49
doi: 10.1093/emboj/17.1.37