Macrophage and dendritic cell subset composition can distinguish endotypes in adjuvant-induced asthma mouse models.

Animals Asthma / immunology Dendritic Cells / cytology Disease Models, Animal Leukocyte Count Macrophages / cytology Mice Neutrophils / immunology

Journal

PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081

Informations de publication

Date de publication:
2021
Historique:
received: 01 04 2021
accepted: 18 05 2021
entrez: 1 6 2021
pubmed: 2 6 2021
medline: 21 10 2021
Statut: epublish

Résumé

Asthma is a heterogeneous disease with neutrophilic and eosinophilic asthma as the main endotypes that are distinguished according to the cells recruited to the airways and the related pathology. Eosinophilic asthma is the treatment-responsive endotype, which is mainly associated with allergic asthma. Neutrophilic asthma is a treatment-resistant endotype, affecting 5-10% of asthmatics. Although eosinophilic asthma is well-studied, a clear understanding of the endotypes is essential to devise effective diagnosis and treatment approaches for neutrophilic asthma. To this end, we directly compared adjuvant-induced mouse models of neutrophilic (CFA/OVA) and eosinophilic (Alum/OVA) asthma side-by-side. The immune response in the inflamed lung was analyzed by multi-parametric flow cytometry and immunofluorescence. We found that eosinophilic asthma was characterized by a preferential recruitment of interstitial macrophages and myeloid dendritic cells, whereas in neutrophilic asthma plasmacytoid dendritic cells, exudate macrophages, and GL7+ activated B cells predominated. This differential distribution of macrophage and dendritic cell subsets reveals important aspects of the pathophysiology of asthma and holds the promise to be used as biomarkers to diagnose asthma endotypes.

Identifiants

DOI: 10.1371/journal.pone.0250533 PMID: 34061861 PMC: PMC8168852
pubmed: 34061861
doi: 10.1371/journal.pone.0250533
pii: PONE-D-21-10740
pmc: PMC8168852
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0250533

Déclaration de conflit d'intérêts

The authors have declared that no competing interests exist.

Références

Mucosal Immunol. 2010 Nov;3(6):537-44
pubmed: 20811344
J Immunol. 2008 Feb 15;180(4):2562-72
pubmed: 18250467
Infect Immun. 2010 Jun;78(6):2620-30
pubmed: 20368349
J Allergy Clin Immunol. 2018 Mar;141(3):893-905.e6
pubmed: 28579374
Environ Health Perspect. 2014 Jan;122(1):34-42
pubmed: 24168764
Thorax. 2002 Aug;57(8):677-82
pubmed: 12149526
Immunity. 2016 Mar 15;44(3):439-449
pubmed: 26982352
Methods Cell Biol. 2004;75:241-56
pubmed: 15603429
Am J Respir Cell Mol Biol. 2017 Sep;57(3):294-306
pubmed: 28421818
J Allergy Clin Immunol Pract. 2019 May - Jun;7(5):1507-1513.e1
pubmed: 30654200
Allergy. 2012 Jul;67(7):835-46
pubmed: 22594878
J Exp Med. 2000 Sep 4;192(5):741-54
pubmed: 10974039
J Exp Med. 2004 Jul 5;200(1):89-98
pubmed: 15238608
Eur Respir J. 2003 Aug;22(2):374-82
pubmed: 12952276
J Immunol. 2017 Dec 15;199(12):3943-3951
pubmed: 29093062
J Allergy Clin Immunol. 2009 May;123(5):1185-7
pubmed: 19361847
J Exp Med. 2011 Jun 6;208(6):1151-62
pubmed: 21624935
Allergy. 2013 Jun;68(6):695-701
pubmed: 23662841
Eur Respir Rev. 2015 Dec;24(138):594-601
pubmed: 26621973
J Allergy Clin Immunol. 2010 Dec;126(6):1081-91; quiz 1092-3
pubmed: 20709383
Thorax. 2007 Feb;62(2):168-75
pubmed: 16928719
Clin Exp Allergy. 2002 Jun;32(6):818-23
pubmed: 12047425
Eur Respir J. 2002 May;19(5):879-85
pubmed: 12030728
Clin Exp Allergy. 2018 May;48(5):502-512
pubmed: 29431885
J Allergy Clin Immunol. 2018 Apr;141(4):1395-1410
pubmed: 28889953
J Immunol. 2013 Apr 1;190(7):3447-57
pubmed: 23467934
Mucosal Immunol. 2015 Jul;8(4):863-73
pubmed: 25465099
Eur Respir J. 2017 May 1;49(5):
pubmed: 28461300
Allergol Int. 2017 Jul;66(3):369-376
pubmed: 28391979
Eur Respir J. 2018 Mar 1;51(3):
pubmed: 29496789
Eur Respir J. 2018 Mar 1;51(3):
pubmed: 29496785
Thorax. 2007 Mar;62(3):211-8
pubmed: 16844729
J Allergy Clin Immunol. 2014 May;133(5):1280-8
pubmed: 24589344
J Exp Med. 2017 Aug 7;214(8):2387-2404
pubmed: 28694385
J Immunol. 2015 Oct 15;195(8):3838-48
pubmed: 26355152
Cell Mol Immunol. 2015 Mar;12(2):202-12
pubmed: 25363529
Clin Exp Allergy. 2019 Jan;49(1):44-53
pubmed: 30107059
J Exp Med. 2010 Sep 27;207(10):2097-111
pubmed: 20819925
Allergy. 2020 Feb;75(2):311-325
pubmed: 31309578
Inflamm Res. 2020 Jan;69(1):139-151
pubmed: 31820024
Immunity. 2017 Mar 21;46(3):457-473
pubmed: 28329706
Am J Physiol Lung Cell Mol Physiol. 2011 May;300(5):L679-90
pubmed: 21335522
Clin Exp Allergy. 2016 Jan;46(1):60-70
pubmed: 26767492
J Allergy Clin Immunol. 2019 Nov;144(5):1428-1432.e8
pubmed: 31369802
Am J Respir Crit Care Med. 2012 Mar 15;185(6):612-9
pubmed: 22268133
Sci Rep. 2018 May 2;8(1):6925
pubmed: 29720689
Am J Pathol. 2011 Jan;178(1):198-211
pubmed: 21224057
Methods Mol Biol. 2017;1559:121-136
pubmed: 28063042
J Allergy Clin Immunol. 2014 Feb;133(2):388-94
pubmed: 24075231
Am J Respir Cell Mol Biol. 2008 Jan;38(1):105-13
pubmed: 17690327
Immunol Rev. 2017 Jul;278(1):63-86
pubmed: 28658551
J Immunol. 2012 Feb 15;188(4):1809-18
pubmed: 22250079
Mucosal Immunol. 2015 Nov;8(6):1212-25
pubmed: 25760421
Sci Rep. 2016 Aug 16;6:31663
pubmed: 27526865
Nat Med. 2006 Dec;12(12):1345-6; author reply 1347
pubmed: 17151684
Am J Respir Crit Care Med. 2011 Feb 1;183(3):299-309
pubmed: 20813890
Immunity. 2016 Dec 20;45(6):1299-1310
pubmed: 28002730
Nat Immunol. 2015 Jan;16(1):45-56
pubmed: 25521684
Am J Respir Cell Mol Biol. 2006 Aug;35(2):227-35
pubmed: 16543608
J Allergy Clin Immunol. 2010 Jul;126(1):133-9
pubmed: 20538329
Exp Ther Med. 2018 Mar;15(3):2492-2498
pubmed: 29456654
Chest. 2020 Jan;157(1):26-33
pubmed: 31525357
J Immunol. 2014 Oct 15;193(8):4245-53
pubmed: 25225663
Nat Immunol. 2019 Nov;20(11):1444-1455
pubmed: 31591573
Lancet. 2018 Jan 27;391(10118):350-400
pubmed: 28911920
J Immunol. 2019 May 1;202(9):2519-2526
pubmed: 31010841
J Immunol. 2009 Jul 15;183(2):1074-82
pubmed: 19553531
Am J Respir Cell Mol Biol. 2017 Jul;57(1):66-76
pubmed: 28257233
Mucosal Immunol. 2015 May;8(3):444-63
pubmed: 25669149
Inflamm Regen. 2018 Jun 20;38:10
pubmed: 29951134
Chest. 2001 May;119(5):1329-36
pubmed: 11348936
Trends Immunol. 2017 Dec;38(12):942-954
pubmed: 28784414
J Immunol. 2003 Aug 15;171(4):1637-41
pubmed: 12902459
Am J Respir Crit Care Med. 2009 Oct 15;180(8):720-30
pubmed: 19661246
Immunology. 2020 Jun;160(2):126-138
pubmed: 31715003
Proc Natl Acad Sci U S A. 2007 Sep 11;104(37):14765-70
pubmed: 17766435
Nat Med. 2003 May;9(5):582-8
pubmed: 12669034
Eur Respir Rev. 2019 Oct 21;28(153):
pubmed: 31636089
J Trauma Acute Care Surg. 2014 Apr;76(4):982-90
pubmed: 24662861
J Allergy Clin Immunol. 2020 Feb;145(2):496-498
pubmed: 31812572
Nat Protoc. 2015 May;10(5):792-806
pubmed: 25927389
Trends Mol Med. 2016 Apr;22(4):303-316
pubmed: 26979628
J Exp Med. 2018 Feb 5;215(2):537-557
pubmed: 29273643
Allergy. 2013 Feb;68(2):180-9
pubmed: 23205591

Auteurs

Müge Özkan (M)

Department of Genome Sciences and Molecular Biotechnology, Izmir International Biomedicine and Genome Institute, Dokuz Eylul University, Balcova/Izmir, Turkey.
ORCID: 0000-0002-3927-1882

Yusuf Cem Eskiocak (YC)

Izmir Biomedicine and Genome Center (IBG), Balcova/Izmir, Turkey.

Gerhard Wingender (G)

Izmir Biomedicine and Genome Center (IBG), Balcova/Izmir, Turkey.
Department of Biomedicine and Health Technologies, Izmir International Biomedicine and Genome Institute, Dokuz Eylul University, Balcova/Izmir, Turkey.
ORCID: 0000-0002-7115-298X

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Macrophage and dendritic cell subset...
questionsmedicales.fr › Maladies du système immunitaire › Hypersensibilité › Hypersensibilité immédiate › Hypersensibilité respiratoire › Asthme › Macrophage and dendritic cell subset...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Système phagocytaire mononucléé › Cellules dendritiques › Macrophage and dendritic cell subset...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles biologiques › Modèles animaux de maladie humaine › Macrophage and dendritic cell subset...
questionsmedicales.fr › Phénomènes physiologiques respiratoires et circulatoires › Phénomènes physiogiques du sang › Hémogramme › Numération des leucocytes › Macrophage and dendritic cell subset...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Phagocytes › Macrophages › Macrophage and dendritic cell subset...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Macrophage and dendritic cell subset...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Phagocytes › Granulocytes neutrophiles › Macrophage and dendritic cell subset...